Effects of Baihe Dihuang Decoction on Intestinal Flora of Depressed Rats
Objective:To explore the effect of Baihe Dihuang Decoction on the behavior and intestinal flora of depressed rats.Methods:The rat model of depression was prepared by solitary feeding and chronic unpredictable mild stress stimulation.After ad-ministration of Baihe Dihuang Decoction,the pharmacodynamics were evaluated by sugar water preference test and open field test,and the colonic pathology of rats was examined.Meanwhile,rat feces were collected,and 16S rRNA high-throughput sequencing technology was used to analyze the diversity of rat intestinal flora and predict its function.Results:Compared to the normal group,the weight of rats in the model group decreased significantly.After administration,the weight of rats in each dose group had a tend-ency to increase.The results of sugar water preference experiment showed that the sugar water preference of rats in the model group was significantly weakened compared to the normal group(P＜0.01).After administration,the preference for sugar water in all dose groups increased,and the difference between medium dose group and low dose group was statistically significant(P＜0.01).In an open field experiment,compared to the normal group,the autonomic activity of rats in the model group significantly decreased(P＜0.01).After administration,the autonomous activity of rats in all dose groups had a trend of enhancement,and the difference between the low-dose group and the model group was statistically significant(P＜0.05).The pathological results showed that the colonic mucosa of the model group was destroyed,and the colonic mucosa of each administration group recovered to different de-grees after receiving Baihe Dihuang Decoction.In terms of bacterial diversity,after treatment with Baihe Dihuang Decoction,the a-bundance of Firmicutes increased,and the abundance of Bacteroidota,Verrucomicrobia,Proteobacteria and Cyanobacteria de-creased.The changes of Proteobacteria and Cyanobacteria were statistically significant(P＜0.05).At the genus level,the abun-dances of genera such as norank_f__Muribaculaceae,Ruminococcus and Faecalibacterium decreased,and the abundance of genera such as Lactobacillus,Rombutzia,Turicibacter and Bacillus increased.The changes in norank_f__Muribaculaceae,Rombutzia,Tu-ricibacter,norank_f__Eubacterium_coprostanoligenes_group,Prevotellaceae_UCG-001 and Prevotellaceae_UCG-001 were statisti-cally significant(P＜0.05).KEGG function prediction showed that the function of different flora was related to amino acid metabo-lism,energy metabolism,nervous system and neurodegenerative diseases.Conclusion:Baihe Dihuang Decoction can improve the depression-like behavior of depressed rats by regulating the intestinal flora of depressed rats.
万方数据
维普
