Objective:To observe the protective effect of gentiopicroside on cartilage extracellular matrix in osteoarthritis and ex-plore its mechanism.Methods:Cartilage cells were extracted from articular cartilage and cultured in vitro until primary generation.The experiment set up blank control group,model group and observation group.The control group used conventional culture medi-um.The model group had interleukin-1 β(IL-1 β)added to the culture medium to induce an osteoarthritis model at the cellular lev-el.The observation group received gentiopicroside intervention on the basis of the model group conditions.RNA was extracted after 72 h of co-culture in vitro.Real-time fluorescent quantitative reverse transcription polymerase chain reaction(RT-PCR)was used to detect the expression changes of specific genes for articular cartilage extracellular matrix,such as type Ⅱ collagen A1,proteogly-can,matrix metalloproteinase-13(MMP-13),and a disintegrin and metalloproteinase with thrombospondin motifs-5.(ADAMTS-5.)Results:Compared with the control group,the expression of type Ⅱ collagen A1 and proteoglycan in the model group decreased sig-nificantly(P＜0.01),and the expressions of MMP-13 and ADAMTS-5 increased significantly(P＜0.01).Compared with the mod-el group,the expressions of type Ⅱ collagen A1 and proteoglycan in the observation group increased,with the difference in type Ⅱcollagen A1 being statistically significant(P＜0.05),and the expressions of MMP-13 and ADAMTS-5 decreased,with statistically significant differences(P＜0.05).Conclusion:Gentiopicroside can upregulate the mRNA expression of type Ⅱ collagen A1 and proteoglycan in the extracellular matrix of cartilage,and has a protective effect on the cartilage cells in osteoarthritis,primarily by inhibiting the production of MMP-13.
维普
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