Effects of Hedysarum Polysaccharides on Apoptosis of Bladder Cancer Cells Mediated by Mitochondrial Pathway
Objective:To observe the effects of Hedysarum polysaccharides(HPS)on the apoptosis of bladder cancer cells media-ted by mitochondrial pathway,and to explore the possible mechanism.Methods:T24 cells in log phase were collected and randomly divided into control group(conventional culture),Hedysarum polysaccharides group(adding Hedysarum polysaccharides 0.8 μg/L),SP600125 group(adding SP600125 10 μmol/L),and combination group(adding Hedysarum polysaccharides)0.8 μg/L,SP600125 10 μmol/L).MTT assay was used to detect cell proliferation.Double staining was used to detect the apoptosis rate.JC-1 method was used to detect mitochondrial damage in cells.Western blotting was used to detect expressions of cell-related proteins.Results:Compared with the control group,the absorbance values at 24,48 and 72 h,mitochondrial membrane potential,Bcl-2 pro-tein expression were decreased,apoptosis rate,expressions of Bax and Caspase-3 proteins,p-JNK/JNK,p-c-Jun/c-Jun were in-creased in the HPS group(P＜0.05),and the absorbance values at 24,48 and 72 h,mitochondrial membrane potential,Bcl-2 pro-tein expression were increased,apoptosis rate,expressions of Bax and Caspase-3 proteins,p-JNK/JNK,p-c-Jun/c-Jun were de-creased in the SP600125 group(P＜0.05).Compared with the HPS group,the absorbance values at 24,48 and 72 h,mitochondrial membrane potential,and Bcl-2 protein expression were increased,and apoptosis rate,expressions of Bax and Caspase-3 proteins,p-JNK/JNK,p-c-Jun/c-Jun were decreased in the combination group(P＜0.05).Conclusion:HPS can inhibit the proliferation of T24 cells and promote their apoptosis by mediating the mitochondrial pathway.The mechanism may be related to the activation of the JNK/c-Jun signaling pathway.
Hedysarum polysaccharidesMitochondrionBladder cancerApoptosisBcl-2-related X proteinc-Jun N-terminal kinase(JNK)Caspase-3
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