大鼠急性血瘀证模型造模方法的网状Meta分析
Network Meta-Analysis of Modeling Methods for Rats with Acute Blood Stasis Syndrome
宋慧平 1岳傲春 2李泽坤 2韩伟 3李琴4
作者信息
- 1. 山东中医药大学第一临床学院,济南,250199;深圳大学总医院急诊科,深圳,518071
- 2. 青岛大学医学部,青岛,266075
- 3. 深圳大学总医院急诊科,深圳,518071
- 4. 深圳大学总医院急诊科,深圳,518071;青岛大学附属烟台毓璜顶医院中西医结合科,烟台,264099
- 折叠
摘要
目的:采用网状Meta分析评价不同急性血瘀证模型造模方法的效果,为中医实验研究急性血瘀证动物模型的制备提供循证依据.方法:检索中国期刊全文数据库(CNKI)、中国学术期刊数据库(CSPD)、中文科技期刊数据库(CCD)中有关急性血瘀证模型的建模方法,应用Stata软件进行网状Meta分析.结果:最终纳入32项研究,共纳入9种造模方案.各项指标最优前3位概率排序分别为:1)全血黏度:1 S-1切变率右旋糖酐＞高脂＞氢化可的松+肾上腺素,10 S-1切变率冰水浴＞肾上腺素＞高脂+力竭游泳,30 S-1切变率多因素刺激＞右旋糖酐＞肾上腺素,60 S-1切变率多因素刺激＞右旋糖酐＞肾上腺素,150 S-1切变率肾上腺素＞冰水浴＞高脂+力竭游泳,200 S-1切变率右旋糖酐＞氢化可的松+肾上腺素＞角叉莱胶+内毒素;2)血浆黏度:右旋糖酐＞多因素刺激＞肾上腺素;3)红细胞聚集指数:冰水浴＞多因素刺激＞高脂+力竭游泳.受纳入研究质量和数量的限制,所得结论仍待进一步验证.结论:右旋糖酐、冰水浴、多因素刺激、肾上腺素造模效果更显著.
Abstract
Objective:To evaluate the effect of different modeling methods for acute blood stasis syndrome by network meta-analy-sis and provide an evidence-based basis for the preparation of an animal model with acute blood stasis syndrome in research of tra-ditional Chinese medicine(TCM).Methods:The modeling methods for acute blood stasis syndrome were searched from Databases of China National Knowledge Infrastructure(CNKI),China Science Periodical Database(CSPD),and Chinese Citation Database(CCD).The network meta-analysis was carried out by Stata software.Results:A total of 32 pieces of literature and nine modeling schemes were finally included.The best first three probability indexes were ranked as follows:1)whole blood viscosity:1 S-1 shear rate dextran＞high fat＞hydrocortisone+adrenaline,10 S-1 shear rate ice water bath＞adrenaline＞high-fat feeding+exhaustive swimming,30 S-1 shear rate multifactor stimulation＞dextran＞adrenaline,60 S-1 shear rate multifactor stimulation＞dextran＞adrenaline,150 S-1 shear rate adrenaline＞ice water bath＞high-fat feeding+exhaustive swimming,200 S-1 shear rate dextran＞hydrocortisone+adrenaline＞carrageenan+endotoxin;2)plasma viscosity:dextran＞multifactor stimulation＞adrenaline;3)e-rythrocyte aggregation index:ice water bath＞multifactor stimulation＞high-fat feeding+exhaustive swimming.Limited by the quali-ty and quantity of the included studies,the conclusions still need to be further verified.Conclusion:dextran,ice water bath,multi-factor stimulation,and adrenaline are more effective in modeling.
关键词
急性血瘀证/动物模型/造模方法/大鼠/模型评价/实验方法/网状Meta分析/系统评价Key words
Acute blood stasis syndrome/Animal model/Modeling methods/Rats/Assessment of model/Experimental methods/Network meta-analysis/Systematic review引用本文复制引用
基金项目
国家自然科学基金项目(82071465)
国家自然科学基金项目(81571283)
山东省自然科学基金项目(ZR2020MH154)
山东省自然科学基金项目(ZR2016HL21)
山东省重点研发项目(2018GSF118215)
深圳市医疗卫生三名工程项目(SZSM201911007)
出版年
2024
国家科技期刊平台
NSTL
万方数据