摘要
目的:研究麒麟竭Daemonorops draco Bl.果实乙醇提取物(血竭)的乙酸乙醋提取部位中化学成分及其抑制血小板活化活性.方法:采用硅胶、ODS、LH-20凝胶柱色谱和半制备高效液相色谱技术对麒麟竭果实乙醇提取物的乙酸乙酯部位的化学成分进行分离纯化,结合1H-NMR、13C-NMR、MS等波谱学数据进行结构鉴定,采用凝血酶诱导的血小板活化释放ATP模型对化学成分进行活性筛选.结果:从麒麟竭果实乙醇提取物的乙酸乙酯提取部位中分离得到11个化合物.分别鉴定为 Ethyl 4,6-dihydroxy-2-methoxy-3-methylbenzoate(1)、6-寒基-2,4-二甲氧基-3-甲基苯乙醇(2)、4,6-二羧基-2-甲氧基-3-甲基苯甲醛(3)、对羟基苯甲酸乙酯(4)、4-羟基苯甲酸甲酯(5)、3,5-二氯水杨酸(6)、对羟基苯甲醛(7)、Pinocem-brin(8)、Strobopinin(9)、7-羟基-5-甲氧基-6-甲基二氢黄酮(10)和Ozoroalide(11).结论:化合物1为新化合物,化合物2和3为新天然产物.化合物3、7和9可以明显地抑制凝血酶诱导的血小板活化释放ATP.
Abstract
Objective:To investigate the chemical constituents in the ethyl acetate fraction of the fruit extract of Daemonorops draco Bl.and their platelet-inhibitory activities.Methods:The ethyl acetate fraction of the fruit extract of D.draco was separated and pu-rified by column chromatography with silica gel,ODS,and Sephadex LH-20 and semi-preparative HPLC.The structures of the chemical constituents were identified based on the spectral data from 1 H-NMR,l3C-NMR,and MS.The ATP release from thrombin-activated platelets was employed to screen the active constituents.Results:Eleven compounds were obtained from the ethyl acetate fraction of the fruit extract of D.draco.They were identified as ethyl 4,6-dihydroxy-2-methoxy-3-methylbenzoate(1),6-hydroxy-2,4-dimethoxy-3-methyl phenylethanol(2),4,6-dihydroxy-2-methoxy-3-methyl benzaldehyde(3),ethyl p-hydroxy benzoate(4),methyl 4-hydroxy benzoate(5),3,5-dichlorosalicylic acid(6),p-hydroxybenzaldehyde(7),pinocembrin(8),strobopinin(9),7-hy-droxy-5-methoxy-6-methylflavanone(10),and ozoroalide(11).Conclusion:Compound 1 was a new compound and compounds 2 and 3 were new natural products.Compounds 3,7,and 9 significantly inhibited the ATP release from thrombin-activated platelets.
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