首页|鼻敏宁颗粒对寒证变应性鼻炎大鼠的作用机制研究

鼻敏宁颗粒对寒证变应性鼻炎大鼠的作用机制研究

扫码查看
原文链接
  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
目的:探索鼻敏宁颗粒对寒证变应性鼻炎大鼠鼻黏膜组织中黏蛋白5B(MUC5B)、黏蛋白5AC(MUC5AC)、Toll样受体2(TLR2)蛋白表达情况.方法:将60只大鼠随机分成空白对照组、模型组、阳性对照组、鼻敏宁低、中、高剂量组.造模成功后,鼻敏宁颗粒高剂量组大鼠予鼻敏宁颗粒2 g/kg,鼻敏宁颗粒中剂量组1 g/kg,鼻敏宁颗粒低剂量组0.5 g/kg,阳性对照组予辛芩颗粒5 g/kg,2 mL/次,空白对照组与模型组给予同等容积的0.9％氯化钠注射液灌胃,均为每天1次,连续7 d.应用免疫组织化学定位检测鼻黏膜上皮细胞TLR2蛋白表达情况,应用蛋白质印迹法(WB)检测鼻黏膜上皮组织MUC5B及MUC5AC蛋白表达量.结果:鼻敏宁低中高剂量组中阳性染色明显减弱,模型组与其他组比较,差异有统计学意义(P＜0.05);与模型组比较,中剂量组与高剂量组MUC5B及MUC5AC含量显著降低,有明显的差异有统计学意义(P＜0.05).结论:鼻敏宁颗粒可通过降低黏蛋白5B及黏蛋白5AC的表达,TLR2蛋白的表达,减轻大鼠鼻黏膜免疫损伤,达到治疗变应性鼻炎的作用.
Mechanism of Biminning Granules in Treating Allergic Rhinitis in Rats with Cold Syndrome
Objective:To explore the expression of mucin 5B(MUC5B),mucin 5AC(MUC5AC),and Toll-like receptor 2(TLR2)in the nasal mucosa of the rats with allergic rhinitis(AR)due to cold syndrome and treated with Biminning Granules.Methods:Sixty rats were randomized into 6 groups:blank control,model,positive control(Xinqin Granules,5 g/kg,2 mL per time),and low-,medium-,and high-dose(0.5,1,and 2 g/kg,respectively)Biminning Granules.The successfully modeled rats were administrated with corresponding drugs or 0.9％sodium chloride(blank control and model groups)by gavage once a day for 7 days.The immu-nohistochemical assay was employed to localize the expression of TLR2 in the nasal mucosa.Western blotting was employed to de-termine the expression levels of MUC5B and MUC5AC in the nasal mucosa.Results:Positive staining was significantly attenuated in the Biminning groups,and the model group showed significant differences compared with other groups(P＜0.05).Compared with the model group,medium and high-dose Biminning groups showcased down-regulated expression of MUC5B and MUC5AC(P＜0.05).Conclusion:Biminning Granules can reduce the immune damage to the nasal mucosa by down-regulating the expres-sion of MUC5B,MUC5AC,and TLR2,thereby treating AR in rats.

Allergic rhinitisBiminning GranulesImmunohistochemistryAnimal experimentToll-like receptor 2(TLR2)Mu-cin 5B(MUC5B)Mucin 5AC(MUC5AC)Western blotting

李欣宇、钟常英、彭波、苏卓异、徐慧贤

展开 >

湖南中医药高等专科学校,株洲,412012

湖南省中医药研究院附属医院,长沙,410006

长春中医药大学,长春,130117

广州中医药大学第一附属医院耳鼻喉科,广州,510405

展开 >

变应性鼻炎 鼻敏宁颗粒 免疫组化 动物实验 Toll样受体2 黏蛋白5B 黏蛋白5AC 蛋白质免疫印迹法

国家自然科学基金面上项目湖南省中医药科研计划项目

82274590202026

2024

10.3969/j.issn.1673-7202.2024.10.007

世界中医药
世界中医药学会联合会

世界中医药

CSTPCDCHSSCD北大核心
影响因子：1.266
ISSN：1673-7202
年,卷(期)：2024.19(10)
  • 21