Effects of Zhonghua Dieda Pills on Promoting Blood Circulation and Resolving Blood Stasis
Objective:To observe the effects of Zhonghua Dieda Pills on in vitro thrombosis formation,blood viscosity,platelet ag-gregation rate in acute blood stasis model rats,and on ear microcirculation in microcirculation disorder model mice to evaluate the blood-activating and stasis-resolving effects of Zhonghua Dieda Pills.Methods:An acute blood stasis model in rats was established by injecting adrenaline and cold water stimulation,and in vitro thrombosis formation and blood viscosity were measured.Platelet ag-gregation rate in rats was measured using the Born turbidimetric method with an inducer.Microcirculation disorder was induced in mice by tail vein injection of 15%high-molecular dextran,and changes in blood flow velocity and vessel diameter of arterioles and venules in the mouse ear were observed over time.Results:Zhonghua Dieda Pills significantly reduced blood viscosity in blood sta-sis model rats,with significant reductions in whole blood viscosity at shear rates of 150 S-1,60 S-1,and 5 S-1(P<0.05).Zhong-hua Dieda Pills significantly shortened thrombus length and reduced both wet and dry weights of thrombi in blood stasis model rats(P<0.05,P<0.01).Zhonghua Dieda Pills inhibited platelet aggregation,with all doses significantly reducing ADP-induced platelet aggregation rates in rats(P<0.05,P<0.01).Additionally,Zhonghua Dieda Pills significantly improved microcirculation disorder induced by high-molecular dextran in mice,increased blood flow velocity and expanded arteriole and venule diameters at 10,20,and 30 min post-modeling(P<0.05,P<0.01),and reduced red blood cell aggregation(P<0.05,P<0.01).Conclu-sion:Zhonghua Dieda Pills can significantly improve blood viscosity,inhibit platelet aggregation,and ameliorate microcirculation disorders,demonstrating a good effect in activating blood circulation and resolving blood stasis.
Zhonghua Dieda PillsPromoting blood circulation and resolving blood stasisBlood stasis modelMicrocirculation disorderPlatelet aggregationBlood viscosityThrombosisExperimental study