Intervention of Yixiao Formula in Diabetes Combined with Fatty Liver Through Transforming Growth Factor-β,Signaling Pathway
Objective:To explore the effects and mechanisms of Yixiao Formula on diabetes combined with fatty liver.Methods:Twelve-week-old male spontaneous diabetic(KKAy)mice were randomly divided into a model group,a Chinese medicine(Yixiao Formula)intervention group,and a Western medicine(losartan)intervention group using a random number table.The control group consisted of age-matched male inbred(C57BL/6J)mice.The intervention period lasted 12 weeks.Changes in blood glucose,blood lipids,and liver function were observed before and after the intervention.Liver tissue was pathologically stained,and changes in pro-teins and mRNAs in transforming growth factor-β1(TGF-β1)signaling pathway in liver tissues were detected.Results:Compared to the model group,the Yixiao Formula group showed a significant reduction in body weight and liver index after 8 weeks of interven-tion(P<0.05,P<0.01).Fasting blood glucose levels significantly decreased at 4,8,and 12 weeks of intervention(P<0.01).Af-ter 8 weeks of intervention,cholesterol,triglycerides,low-density lipoprotein cholesterol,and alanine aminotransferase levels were reduced(P<0.05,P<0.01).Pathologically,the steatosis score decreased after 4 weeks of Yixiao Formula intervention(P<0.05).After 12 weeks of intervention,the protein and mRNA expression of TGF-β1 was significantly downregulated(P<0.05,P<0.01).Conclusion:Yixiao Formula can significantly improve obesity and glucose-lipid metabolism disorders in KKAy mice,regu-late protein and gene expression in TGF-β1 signaling pathway,and reduce liver steatosis.
Yixiao FormulaDiabetesFatty liverGlucose and lipid metabolismTGF-β1/Smads signaling pathwayLosartanKKAy miceExperimental research