Establishment of a Rat Model with Syndrome of Kidney Deficiency and Blood Stasis and Characterization of Kidney-tonifying,Essence-and Blood Activating Effects of Bushen Huoxue Formula
Objective:To establish a rat model with the syndrome of kidney deficiency and blood stasis and study the kidney-toni-fying,essence-replenishing,blood-activating,and stasis-resolving effects of Bushen Huoxue Formula based on the correspondence between syndrome and formula.Methods:A total of 48 female SD rats were randomized into normal,model,positive drug,and low-(0.72 g/kg),medium-(1.44 g/kg),and high-dose(2.89 g/kg)Bushen Huoxue Formula groups(n=8).The rats in other groups except the normal group were administrated with hydroxyurea solution(450 mg/kg)by gavage from the morning of day 1 for 14 con-secutive days.From the morning of day 4,epinephrine[0.3 mg/(kg·d)]was injected subcutaneously until day 14 for the model-ing of kidney deficiency and blood stasis.The general state of the rats was observed,and the uterus and ovary indexes were calculat-ed.Meanwhile,the number of follicles,number of corpus luteum,serum levels of estrodiol(E2),follicle-stimulating hormone(FSH),inhibin B(INHB),red blood cells(RBC),hemoglobin(HGB),hematocrit(HCT),thromboxane B2(TXB-2),endothelin(ET-1),plasma hematocrit,erythrocyte aggregation index,and erythrocyte deformity index were counted and determined.Results:Compared with the normal group,the model group showed decreased uterus index and ovary index(P＜0.001),reduced number of follicles(P＜0.05),increased number of corpus luteum(P＜0.05),lowered serum E2 and FSH levels,elevated INHB level(P＜0.001),declined RBC,HGB,and HCT levels(P＜0.001),increased HCT,erythrocyte aggregation index,and erythrocyte deformity index(P＜0.01,P＜0.001),and risen serum ET-1 and TXB-2 levels(P＜0.001).Compared with the model group,drug interven-tions increased the ovary index(P＜0.01,P＜0.001)and the uterus index(P＜0.05,except the high-dose Bushen Huoxue For-mula group).Drug interventions increased the number of follicles(P＜0.05,P＜0.01,P＜0.001),and Bushen Huoxue Formula decreased the number of corpus luteum(P＜0.05).In addition,drug interventions elevated the serum E2 and FSH levels(P＜0.01,P＜0.001)and lowered the INHB level(P＜0.001),and Bushen Huoxue Formula at different doses increased the RBC,HGB,and HCT.Drug interventions lowered the serum levels of ET-1 and TXB-2(P＜0.001)and decreased HCT(P＜0.001),e-rythrocyte aggregation index(P＜0.001),and erythrocyte deformation index(P＜0.05,except the high-dose Bushen Huoxue For-mula group).Conclusion:For the first time,the rat model with the syndrome of kidney deficiency and blood stasis was established by intragastric administration of hydroxyurea combined with subcutaneous injection of adrenaline.Bushen Huoxue Formula can im-prove the ovarian function and restore the hormone levels to exert the effects of tonifying kidney,replenishing essence,and tonifying blood.Moreover,it can improve the endothelial cell function and reduce thromboxane to active blood and resolve stasis by changing the rheological abnormalities of blood.
Syndrome of kidney deficiency and blood stasisRat modelCorrespondence between syndrome and formulaBushen Huoxue FormulaEfficacyTonifying kidney and replenishing essenceActivating blood and resolving stasisOvarian function
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