首页|补肾活血汤调控宫腔粘连大鼠内膜纤维化进程的作用机制研究

补肾活血汤调控宫腔粘连大鼠内膜纤维化进程的作用机制研究

扫码查看
原文链接
  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
目的:探究微RNA-655(miR-665)/冷诱导核糖核酸结合蛋白(CIRBP)在宫腔粘连(IUA)子宫内膜纤维化中的作用以及补肾活血汤对其的调控作用.方法:使用miRNA靶基因数据库(TargetScan)分析miR-665和CIRBP的结合情况;通过实时定量聚合酶链反应(qRT-PCR)和蛋白免疫印迹技术检测细胞和动物模型中miR-665和CIRBP的转录和表达水平;借助双荧光素酶报告系统和构建过表达载体验证miR-665和CIRBP之间相互调控的作用,以及补肾活血汤对miR-665/CIRBP的调控作用;利用酶联免疫吸附测定(ELISA)和苏木精-伊红(HE)染色检测大鼠宫腔粘连模型中相关因子水平和组织病变程度.结果:在宫腔粘连模型大鼠组织中,CIRBP转录水平显著上升,而miR-665转录水平显著下降.体外实验显示,miR-665模拟物可降低子宫内膜基质细胞中CIRBP的表达水平.此外,补肾活血汤能够降低宫腔粘连的大鼠模型血清中转化生长因子β1(TGF-β1)、雌二醇(E2)和肿瘤坏死因子-α(TNF-α)的水平.补肾活血汤可以提高miR-665的转录水平并降低CIRBP的表达水平,并减轻大鼠子宫内膜组织的病变程度.结论:补肾活血汤通过调控miR-665/CIRBP途径来减轻宫腔粘连大鼠子宫内膜纤维化程度,为宫腔粘连的治疗提供了新的策略和方向.
Bushen Huoxue Decoction Regulates Endometrial Fibrosis in Rat Model of Intrauterine Adhesions
Objective:To investigate the role of microRNA-665(miR-665)/cold-inducible RNA-binding protein(CIRBP)in endo-metrial fibrosis in intrauterine adhesion(IUA)and the regulatory effect of Bushen Huoxue Decoction.Methods:TargetScan was used to analyze the binding of CIRBP with miR-665.Quantitative real-time PCR and Western blotting were employed to determine the transcription and expression levels of CIRBP and miR-665 in cells and animal models.The dual-luciferase reporter system and overexpression vector were utilized to examine the mutual regulation between CIRBP and miR-665,as well as the regulatory effects of Bushen Huoxue Decoction on miR-665/CIRBP.Enzyme-linked immunosorbent assay and hematoxylin-eosin staining were per-formed to measure the levels of relevant factors and pathological changes in the rat model of IUA.Results:The rat model of IUA showed upregulated transcription level of CIRBP and downregulated transcription level of miR-665.The in vitro experiments showed that miR-665 mimics downregulated the expression level of CIRBP in endometrial stromal cells.Bushen Huoxue Decoction lowered the levels of transforming growth factor-β1(TGF-β1),estradiol(E2),and tumor necrosis factor-α(TNF-α)in the serum of the rat model.Additionally,Bushen Huoxue Decoction upregulated the transcription level of miR-665 while downregulating the expression level of CIRBP,thereby alleviating the pathological changes in the endometrial tissue of the rats.Conclusion:Bushen Huoxue De-coction alleviates endometrial fibrosis in the rat model of IUA by regulating the miR-665/CIRBP pathway,which provides new strat-egies and directions for the treatment of IUA.

Intrauterine adhesionmicroRNA-665Cold-inducible RNA-binding proteinBushen Huoxue DecoctionTransfor-ming growth factor-β1EstradiolTumor necrosis factor-α

冯丹、丑丹、张妍、蔡静

展开 >

武汉市中医医院妇科,武汉,430000

华中科技大学同济医学院附属武汉儿童医院妇产科,武汉,430000

宫腔粘连 微RNA-655 冷诱导核糖核酸结合蛋白 补肾活血汤 转化生长因子β1 雌二醇 肿瘤坏死因子-α

国家自然科学基金青年科学基金项目湖北省卫健委重点项目

82274077MY2021M019

2024

10.3969/j.issn.1673-7202.2024.12.007

世界中医药
世界中医药学会联合会

世界中医药

CSTPCDCHSSCD北大核心
影响因子：1.266
ISSN：1673-7202
年,卷(期)：2024.19(12)