Anti-urate Deposition Effects of Cichorium intybus L.Extract Based on Drosophila Model
Objective:To establish a drosophila model of urate deposition,determine modeling conditions,and preliminarily observe the anti-urate deposition effects of Cichorium intybus L.extract.Methods:Drosophila were fed a 5％yeast extract diet to induce u-rate deposition.The urate deposition status was evaluated using polarized light imaging of the Malpighian tubules and hindgut,hexa-mine silver staining,and measurement of uric acid levels in homogenates.The effects of gender and different modeling times on u-rate deposition were investigated.This model was then used to study the anti-urate deposition effects of C.intybus L.extract.Re-sults:Feeding drosophila with 5％yeast extract successfully induced urate deposition.Male drosophila began to show urate deposi-tion after 3 days of modeling,with most showing urate deposition after 7 days.The uric acid levels in the Malpighian tubules and hindgut homogenates of male drosophila continuously increased over time,with no significant differences between different time points.Female drosophila began to show urate deposition after 10 days of modeling,with most showing urate deposition after 14 days.The uric acid levels in the Malpighian tubules and hindgut homogenates of female drosophila continuously increased over time,with significant differences between different time points(P＜0.001).C.intybus L.extract reduced the polarized light signal in the Malpighian tubules and hindgut of drosophila and significantly lowered uric acid levels in homogenates(P＜0.001).Conclu-sion:Feeding female drosophila with 5％yeast extract for 14 days can reliably induce urate deposition.C.intybus L.extract can ex-ert anti-urate deposition effects in the drosophila model.
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