摘要
目的:观察菊苣根水提物对正常大鼠糖、脂、尿酸代谢及肝肾安全性的影响.方法:SD大鼠随机分为空白对照组(水灌胃),菊苣高剂量、中剂量、低剂量组(10 mg/kg、5 mg/kg、2.5 mg/kg菊苣根水提物),别嘌醇组(20 mg/kg别嘌醇溶液),连续灌胃28 d,动态检测实验大鼠血清葡萄糖(GLU)、总胆固醇(TC)、三酰甘油(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、血清尿酸(SUA)、尿尿酸(UUA)、粪便尿酸(FUA)水平,并计算尿酸清除率(CUUA)及12 h尿尿酸排泄量;检测实验大鼠血清中尿酸代谢相关酶,如黄嘌呤氧化酶(XOD)、腺苷脱氨酶(ADA)水平;检测肝肾功能指标,如谷丙转氨酶(GPT)、谷草转氨酶(GOT)、尿素氮(BUN)、肌酐(Cr),并计算肝脏指数及肾脏指数.结果:实验期间,相较于空白对照组,菊苣各给药组大鼠糖、脂、尿酸代谢相关指标GLU、TC、TG、HDL-C、LDL-C、SUA、XOD、ADA、UUA、FUA水平、CUUA、12 h尿尿酸排泄量,以及肝肾安全相关指标GPT、GOT、BUN、Cr、肝脏指数及肾脏指数均无明显差异.结论:菊苣根水提物不会影响正常大鼠糖、脂及尿酸代谢,且具有较好的肝肾安全性.
Abstract
Objective:To observe the effects of water extract of Cichorium intybus L.on glucose,lipid,and uric acid metabolism,as well as liver and kidney safety in normal rats.Methods:SD rats were randomly divided into a blank control group(water by gavage),high-dose,medium-dose,and low-dose C.intybus L.groups(10,5,and 2.5 mg/kg),and a allopurinol group(20 mg/kg allopurinol so-lution).These treatments were conducted via gavage for 28 consecutive days.Dynamic measurements of serum glucose(GLU),total cholesterol(TC),triglycerides(TG),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C),ser-um uric acid(SUA),urinary uric acid(UUA),and fecal uric acid(FUA)levels were taken.Uric acid clearance rate(CUUA)and 12-hour urinary uric acid excretion were calculated.Enzyme levels in serum related to uric acid metabolism,such as xanthine oxidase(XOD)and adenosine deaminase(ADA),were also measured.Liver and kidney function indicators,including alanine aminotrans-ferase(GPT),aspartate aminotransferase(GOT),blood urea nitrogen(BUN),and creatinine(Cr),were evaluated,and liver and kid-ney indices were calculated.Results:During the experimental period,there were no significant differences between the C.intybus L.groups and the blank control group in terms of glucose,lipid,and uric acid metabolism indicators(GLU,TC,TG,HDL-C,LDL-C,SUA,XOD,ADA,UUA,FUA levels,CUUA,and 12-hour urinary uric acid excretion).Additionally,there were no significant differ-ences in liver and kidney safety indicators(GPT,GOT,BUN,Cr,liver index,and kidney index)among the groups.Conclusion:C.inty-bus L.root water extract does not affect glucose,lipid,or uric acid metabolism in normal rats and exhibits good liver and kidney safety.
基金项目
国家自然科学基金联合基金项目(U20A20406)
北京中医药大学双一流-新药研发项目(90010961020091)
维普
万方数据