Objective:To apply magnetic molecular imprinting technique for the specific adsorption and isolation of gentianoside from the uric acid-lowering Chinese medicine Gentianae Macrophyllae Radix.Methods:Magnetic molecularly imprinted polymers(MIPs)of gentianoside were prepared using gentianoside as the template molecule,methacrylic acid(MAA)as the functional mon-omer,ethylene glycol dimethacrylate(EGDMA)as the cross-linker,azobisisobutyronitrile(AIBN)as the initiator,and methanol as the porogenic agent.The materials were characterized using Fourier-transform infrared spectroscopy(FTIR),scanning electron mi-croscopy(SEM),and transmission electron microscopy(TEM).The adsorption performance was evaluated by plotting kinetic ad-sorption curves and static isotherms.Results:The magnetic MIPs of gentianoside was successfully synthesized,and reached the ad-sorption equilibrium after shaking for 60 min.The adsorption capacity for gentianoside extracted and separated from Gentianae Mac-rophyllae Radix was 6.316 mg/g.Conclusion:Gentianoside in the uric acid-lowering Chinese medicine Gentianae Macrophyllae Radix can be specifically captured by magnetic MIPs of gentianoside.
维普
万方数据