Effect and Mechanism of Escin on Proliferation and Invasion of Colorectal Cancer Cells
Objective:To investigate the effect of escin on the proliferation,migration,and invasion of colorectal cancer cell line HCT116 induced by tri methylamine N-oxide(TMAO)and to explore its mechanism of action.Methods:HCT116 colorectal cancer cells were divided into a control group,a TMAO group,and an experimental group.The control group received no treatment,the TMAO group was induced with 100 µmol/L TMAO,and the experimental group was treated with 100 μmol/L TMAO followed by 10 µmol/L escin.Cell proliferation,migration,and invasion were assessed using the MTT assay,scratch wound healing assay,and Transwell migration and invasion assays,respectively.The expression levels of SRC,pBTK,and pTRIO proteins were measured by Western blotting.Results:TMAO induction enhanced the proliferation,migration,and invasion of HCT116 cells and significantly upregulated the mRNA levels of genes in the SRC-BTK-TRIO pathway.Escin treatment inhibited cancer cell proliferation(P＜0.01),reduced their migration(P＜0.001)and invasion(P＜0.01)capabilities,and downregulated the expression of SRC(P＜0.05),BTK(P＜0.01),and TRIO(P＜0.05)proteins.Conclusion:Escin can significantly inhibit the proliferation,migration,and invasion of HCT116 cells by downregulating the SRC-BTK-TRIO pathway.
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