Objective:To investigate whether it is necessary to remove the basal part of the stem of Scrophularia ningpoensis Hemsl.during the purification process.Methods:The yields,moisture content,and extractive substances of the roots and stem basal parts of S.ningpoensis from different batches after purification were compared.The content of five main active components,i.e.,harpagide,harpagoside,angoroside C,acteoside,and cinnamic acid,were analyzed using high-performance liquid chromatography(HPLC).The Scrophularia polysaccharide content was determined using the phenol-sulfuric acid method,and principal component analysis(PCA)was conducted on the aforementioned components.An inflammation model was established by inducing BV-2 micro-glial cells with lipopolysaccharide(LPS),and the anti-inflammatory activity of the roots and stem basal parts of S.ningpoensis was evaluated by measuring the release of nitric oxide(NO)in the cell supernatant to comprehensively evaluate the necessity of remo-ving basal part of stem of S.ningpoensis.Results:The basal part of stem accounted for 10％to 20％of the total weight of S.ning-poensis.The extractive substances in the roots were higher than those in the basal parts of stem.PCA results showed that the roots and basal parts of stem could be classified into two major groups.The content of harpagide,harpagoside,and cinnamic acid,as well as polysaccharides were higher in the roots,but the basal parts of stem also had significant contributions.The basal parts of stem had higher content of angoroside C and acteoside.There was no statistically significant difference in the anti-inflammatory activity between the roots and basal parts of stem.Conclusion:The traditional practice of removing the basal parts of stem of S.ningpoensis during purification has some scientific basis.However,the basal parts of stem also possess certain medicinal values and should be further explored and utilized,especially in the preparation of extracts.
关键词
玄参/玄参芦头/净制/多组分同时测定/多糖/主成分分析/BV-2细胞/抗炎活性
Key words
Scrophularia ningpoensis/Basal part of stem of Scrophularia ningpoensis/Purification/Multicomponent determination/Polysaccharide/Principal component analysis/BV-2 cells/Anti-inflammatory activity
维普
万方数据