Objective:To predict and analyze the quality markers(Q-Markers)of Zingiberis Rhizoma Recens based on ultra-high-performance liquid chromatography-quadrupole-time-of-flight tandem mass spectrometry(UPLC-Q-TOF-MS/MS)technology and network pharmacology.Methods:UPLC-Q-TOF-MS/MS was used to qualitatively analyze the chemical components of Zingiberis Rhizoma Recens in both positive and negative ion modes.Network pharmacology was employed to analyze the modern pharmacologi-cal mechanisms of traditional effects of Zingiberis Rhizoma Recens,thereby preliminarily exploring Q-Markers of Zingiberis Rhizo-ma Recens.Molecular docking technology was used to verify the binding activity between active components and target proteins.Re-sults:A total of 48 chemical components in Zingiberis Rhizoma Recens were identified through UPLC-Q-TOF-MS/MS,with 33 ac-tive components screened as Q-Marker candidates.Network pharmacology analysis of these 33 active components identified 508 tar-get genes and 157 signaling pathways,such as the phosphatidylinositol 3-kinase/protein kinase B(PI3K/AKT)signaling pathway and the Ras signaling pathway.The study preliminarily explored the pharmacological mechanisms of six compounds,i.e.,8-gin-gerone,6-shogaol,8-gingerol,tetrahydrocurcumin,1-dehydro-8-gingerdione,and(E)-4-(3,7-dimethylocta-2,6-dien-1-yl)-2-me-thoxy phenol,and predicted them as Q-Markers of Zingiberis Rhizoma Recens.Conclusion:This study preliminarily predicted the Q-Markers associated with the traditional effects of Zingiberis Rhizoma Recens.
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