Effects of Yinxu Wei tong Capsules on the Ultrastructure and Inflammatory Mediators of Gastric Mucosa in Rats with Chronic Atrophic Gastritis
Objective:To observe the effects of Yinxu Weitong Capsules on the ultrastructure of gastric mucosa and inflammatory mediators in rats with chronic atrophic gastritis(CAG).Methods:A total of 72 specific pathogen-free(SPF)Sprague-Dawley(SD)rats were divided into a normal group(n=13)and an experimental group(n=59).The experimental group was given N-methyl-N'-nitro-N-nitrosoguanidine in drinking water and intragastric administration,combined with irregular feeding to induce a CAG rat model.The modeled rats were randomly divided into a model group,a vitacoenzyme group,and low-,medium-,and high-dose Yinxu Weitong Capsules groups.The Yinxu Weitong Capsules groups received intragastric administration of Yinxu Weitong Capsules at doses of 205.2 mg/kg,410.4 mg/kg,and 820.8 mg/kg,respectively.The vitacoenzyme group received vitacoenzyme solution at 270 mg/kg,and the normal and model groups received an equivalent dose of saline,all once daily for eight weeks.Hematoxylin-eo-sin(HE)staining was used to observe the pathological changes in the gastric mucosa.Transmission electron microscopy was used to observe ultrastructural changes.Real-time quantitative PCR was used to detect the gene expression of tumor necrosis factor-alpha(TNF-α)and interleukin-1 beta(IL-1β)in gastric tissue.Western blot was used to detect the protein expression of pro-inflamma-tory factors TNF-α and IL-1β and anti-inflammatory factors interleukin-4(IL-4)and interleukin-10(IL-10).Results:Compared with the normal group,HE staining in the model group showed reduced glandular tissue,and transmission electron microscopy showed significant structural damage to parietal and chief cells.Moreover,the expression of TNF-α and IL-1 β was higher(P＜0.05),and the expression of IL-4and IL-10 was lower(P＜0.05).Compared with the model group,the medium-and high-dose Yinxu Weitong Capsules groups and the vitacoenzyme group showed improved glandular tissue in HE staining,restored parietal and chief cell structures in transmission electron microscopy,lower expression of TNF-α and IL-1 β in gastric mucosa(P＜0.05),and higher expression of IL-4 and IL-10(P＜0.05).Conclusion:Yinxu Weitong Capsules significantly promote the recovery of abnor-mal gastric mucosal ultrastructure in CAG rats,inhibit the expression of pro-inflammatory factors TNF-α and IL-1 β,and promote the expression of anti-inflammatory factors IL-4 and IL-10.
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