Effect of Electroacupuncture Stimulation on Motor Function and White Matter Injury in Rats with Intracerebral Hemorrhage
Objective:To observe the effects of electroacupuncture stimulation on motor function and white matter injury(WMI)in rats with intracerebral hemorrhage(ICH).Methods:A total of 36 specific pathogen-free(SPF)rats were randomly divided into sham operation(SHAM)group,ICH group,and ICH+electroacupuncture group,with 12 rats in each group.The ICH group and ICH+electroacupuncture group were used to prepare the right striatal ICH model.The ICH+electroacupuncture group was inter-vened in"Quchi"and"Zusanli"acupoints with electroacupuncture for 30 min each day for seven consecutive days.After seven days,the motor function was evaluated by the rotational angle test and the Rotarod test.Luxol fast blue(LFB)staining and myelin basic protein(MBP)immunofluorescence expression were used to evaluate WMI.Glial fibrillary acidic protein(GFAP)and neuro-nal nuclei(NeuN)immunofluorescence expressions were detected to assess the activation of astrocytes and the injury of neurons.The expression levels of tumor necrosis factor-α(TNF-α)and interleukin-1 β(IL-1 β)were detected by enzyme linked immunosor-bent assay(ELISA).Results:Electroacupuncture treatment decreased the frequency of right turns and increased the retention time of rats with ICH in the Rotarod test(P＜0.05).Electroacupuncture intervention could also reduce myelin destruction,increase the expression of MBP,alleviate activation of astrocytes and injury of neurons,and inhibit the expression of TNF-α and IL-1 β(P＜0.05).Conclusion:Electroacupuncture can reduce the overactivation of astrocytes and neuroinflammation,reduce WMI and injury of neurons in the striatum,and promote the recovery of motor function in rats with ICH.
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