炆何首乌乌发的药效评价及其网络药理学机制研究
Pharmacodynamic Evaluation and Network Pharmacology Mechanism of Stewed Polygoni Multiflori Radix for Hair Blackening
潘福竺 1易斌 2许静 3薛艳华 4陈兰 4张建永 5张毅 3王单单 6王韵旨 6陈明霞 2吴宏伟3
作者信息
- 1. 遵义医科大学药学院,遵义,563099;中国中医科学院中药研究所,北京,100700
- 2. 建昌帮药业有限公司,南城,344700;北京斯利安药业有限公司,北京,100176;漯河市中心医院,漯河,462003
- 3. 中国中医科学院中药研究所,北京,100700
- 4. 建昌帮药业有限公司,南城,344700;漯河市中心医院,漯河,462003
- 5. 遵义医科大学药学院,遵义,563099
- 6. 江西省中药炮制(坟法)重点研究室,南城,344100
- 折叠
摘要
目的:对炆何首乌乌发的药效进行评价,并对其作用机制进行研究.方法:构建小鼠白发模型,通过白发面积在新生毛发区域占比、苏木精-伊红(HE)染色及血浆中酪氨酸酶(TYR)的含量来评价炆何首乌的乌发作用;并利用网络药理学及免疫荧光探讨其作用机制.结果:与假手术组比较,模型组小鼠背部白发面积明显升高,皮肤组织中含黑色素的毛囊数量明显降低,血浆中TYR含量明显降低.与模型组比较,阳性药、制何首乌(中剂量、高剂量)及炆何首乌(低剂量、中剂量、高剂量)均能明显降低小鼠白发面积;制/炆何首乌低剂量、中剂量、高剂量能够明显增加小鼠皮肤组织中黑色素毛囊数量;制/炆何首乌高剂量可以明显提高小鼠血浆中TYR含量.在生药量相同的条件下,与制何首乌比较,炆何首乌中剂量能明显增加黑色素毛囊数量;炆何首乌低剂量、高剂量能明显增加小鼠血浆中TYR含量.网络药理学分析发现,炆何首乌的乌发作用涉及黑色素生成和Wnt信号通路等.免疫荧光结果表明,与假手术组比较,模型组小鼠皮肤组织中淋巴增强结合因子1(LEF1)及TYR表达水平明显降低;炆何首乌组小鼠皮肤组织中LEF1及TYR表达水平均明显增高.结论:炆何首乌对小鼠白发模型具有一定的改善作用,其作用机制可能与上调LEF1及TYR的表达水平有关.
Abstract
Objective:To evaluate the hair-blackening efficacy of stewed Polygoni Multiflori Radix(PMR)and investigate its mechanism of action.Methods:A mouse model of white hair was established,and the hair-blackening effect of stewed PMR were assessed by measuring the area of white hair in new hair growth regions,using hematoxylin-eosin(HE)staining,and analyzing plas-ma tyrosinase(TYR)levels.Network pharmacology and immunofluorescence techniques were employed to explore the mechanism of action.Results:Compared to the sham operation group,the model group exhibited a significant increase in the area of white hair on the back,a notable reduction in melanocyte-containing hair follicles in the skin tissue,and a decrease in plasma TYR levels.Com-pared with the model group,treatment with positive control,processed PMR(medium and high doses),and stewed PMR(low,medi-um,and high doses)significantly reduced the white hair area in mice.Processed and stewed PMR at low,medium,and high doses significantly increased the number of melanocyte-containing hair follicles,while the processed and stewed PMR at a high dose nota-bly increased plasma TYR levels.Under the same dose of crude herb,compared with the processed PMR,the stewed PMR at a me-dium dose significantly increased the number of melanocyte-containing hair follicles,and stewed PMR at both low and high doses increased plasma TYR levels.Network pharmacology analysis indicated that the hair-blackening effect of stewed PMR involved mel-anin production and Wnt signaling pathways.Immunofluorescence results showed that,compared with the sham operation group,the model group had significantly reduced expression levels of lymphoid enhancer-binding factor 1(LEF1)and TYR in skin tissue,while the stewed PMR groups exhibited increased levels of LEF1 and TYR expression.Conclusion:Stewed PMR has a notable effect on improving the white hair model in mice,and its mechanism may be related to the upregulation of LEF1 and TYR expres-sion levels.
关键词
炆制/何首乌/乌发/酪氨酸酶/淋巴增强结合因子1/黑色素生成/Wnt信号通路/氢醌Key words
Stewed/Polygoni Multiflori Radix/Hair-blackening/Tyrosinase/Lymphoid enhancer-binding factor 1/Melanin pro-duction/Wnt signaling pathway/Hydroquinone引用本文复制引用
基金项目
国家自然科学基金青年科学基金项目(82204973)
江西省重点研发计划项目(20223BBG71001)
抚州市"揭榜挂帅"项目(XMBH00031)
中国中医科学院科技创新工程项目(ZZ15-YQ-034)
中国中医科学院科技创新工程项目(CI2021B015)
中国中医科学院科技创新工程项目(ZXKT21030)
出版年
2024
国家科技期刊平台
NSTL
万方数据