基于氨基酸及特征肽测定的不同产地鸡内金质量研究
Quality of Galli Gigerii Endothelium Corneum from Different Origins Based on the Determination of Amino Acids and Characteristic Peptides
李国卫 1胡绮萍 1童培珍 1邱韵静 1曾熙欣 2郭嘉亮 2潘礼业 1何嘉莹 1陈向东 1孙冬梅1
作者信息
- 1. 广东一方制药有限公司/广东省中药配方颗粒企业重点实验室,佛山,528244
- 2. 佛山科学技术学院,佛山,528000
- 折叠
摘要
目的:建立鸡内金氨基酸及特征肽的含量测定方法,对不同产地鸡内金进行质量评价.方法:采用高效液相色谱法对鸡内金的12个氨基酸类成分进行测定,同时采用超高效液相串联三重四级杆质谱法对鸡内金的2个特征肽进行测定,并采用偏最小二乘法分析(PLS-DA)对测定结果进行分析.结果:18批鸡内金的12种氨基酸成分总含量相对标准偏差(RSD)范围为2.84％～14.01％,鸡源多肽Ⅰ、鸡源多肽Ⅱ含量RSD分别为47.13％、14.62％,均呈现一定的波动,PLS-DA分析结果显示,3批产地山西和3批产地辽宁独自分为一类,9批产地山东与3批产地浙江的样品共同分为一类.结论:本研究建立的鸡内金氨基酸及特征肽的含量测定方法,能较好地评价不同产地的鸡内金质量,可为鸡内金及动物类药材的质量评价提供参考.
Abstract
Objective:To establish the content determination method of amino acids and characteristic peptides in Galli Gigerii En-dothelium Corneum(GGEC)and evaluate the quality of GGEC from different origins.Methods:High-performance liquid chroma-tography(HPLC)was used to measure the content of 12 amino acids in GGEC,while ultra-high-performance liquid chromatogra-phy-tandem mass spectrometry(UHPLC-MS/MS)was employed to determine the levels of two characteristic peptides.Partial least squares discriminant analysis(PLS-DA)was used to analyze the results.Results:The relative standard deviation(RSD)for the to-tal content of the 12 amino acids in 18 batches of GGEC ranged from 2.84％to 14.01％.The RSD for the levels of the two charac-teristic peptides,Chicken-derived peptide Ⅰ and Chicken-derived peptide Ⅱ,were 47.13％and 14.62％,respectively,indicating some fluctuations.PLS-DA results showed that three batches of samples from Shanxi and three batches of samples from Liaoning were distinctly grouped into separate categories,while nine batches of samples from Shandong and three batches of samples from Zhejiang were grouped together.Conclusion:The established method for determining amino acids and characteristic peptides in GGEC effectively evaluate the quality of GGEC from different origins and can provide a reference for the quality assessment of GGEC and other animal-based medicinal materials.
关键词
鸡内金/产地/氨基酸/特征肽/含量测定/偏最小二乘法分析/统计学/质量控制Key words
Galli Gigerii Endothelium Corneum/Origin/Amino acid/Characteristic peptide/Content determination/Partial least squares discriminant analysis/Statistics/Quality control引用本文复制引用
基金项目
国家自然科学基金项目(82373835)
广东省基础与应用基础研究基金项目(2020B1515120033)
出版年
2024
国家科技期刊平台
NSTL
万方数据