Anti-inebriation and Hepatoprotective Effects of Renshen Zhijuzi Oral Liquid
Objective:To investigate the effects of Renshen Zhijuzi Oral Liquid(RZOL)on alcohol detoxification and liver protec-tion.Methods:Fifty mice were randomly divided into a blank group,a model group,a high-dose RZOL group,a low-dose RZOL group,and a positive control group(bifendate),with 10 mice in each group.After 4 weeks of oral gavage,an acute alcohol intoxica-tion model was induced using a single dose of 50％ethanol.The effects of RZOL on basic physiological activities and serum and liver tissue indicators were investigated,and pathological sections of liver tissue were observed.The anti-inebriation and hepatopro-tective effects of RZOL were assessed.Additionally,an AML12 liver cell injury model was established with ethanol,and the impact of RZOL on ethanol-stimulated AML12 cell supernatants was measured by examining glutamic-pyruvic transaminase(GPT),glutam-ic oxalacetic transaminase(GOT),and reactive oxygen species(ROS)levels.Results:Mice treated with RZOL had significantly ex-tended intoxication times,shortened sleep time and time to wake-up,significantly reduced serum ethanol concentration,GPT,and GOT levels,improved liver cell damage,increased superoxide dismutase(SOD)activity and glutathione peroxidase(GSH)levels in liver tissue,and reduced malondialdehyde(MDA)content.In AML12 liver cells cultured in vitro,RZOL reversed ethanol-induced increases in ALT and AST activities and excessive ROS production.Conclusion:RZOL demonstrates effective anti-inebriation,de-toxification,and liver protection properties.Its mechanism likely involves enhancing ethanol metabolism and boosting endogenous antioxidant enzyme activity.
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