Effect of Jianpi Zhidong Decoction on the Expression of Histamine and Histamine Decarboxylase in Tourette Syndrome Model Rats
Objective:To investigate the effect of Jianpi Zhidong Decoction on the histamine(HA)and histidine decarboxylase(HDC)expression in the striatum and serum in a Tourette syndrome(TS)rat model.Methods:Twenty-four SD rats were randomly assigned to blank,model,Chinese medicine,and western medicine groups according to a random number table,with six rats in each group.Except for the blank group,all other groups were given IDPN at 250 mg/kg via intraperitoneal injection for 7 days to establish the TS model.The blank and model groups were administered with saline at 15 mL/kg,the Chinese medicine group with Jianpi Zhi-dong Decoction at 16 g/kg,and the western medicine group with sulpiride at 21 mg/kg,once daily,for 6 weeks.Body weight and be-havioral changes of all groups were compared.Enzyme-linked immunosorbent assay(ELISA)was used to measure HA levels in the striatum and serum.Reverse transcription polymerase chain reaction(RT-PCR)was used to detect HDC mRNA expression in the stri-atum.Western blot and immunohistochemistry were used to assess HDC protein expression in the striatum.Results:There were no sig-nificant differences in body weight among the model,Jianpi Zhidong Decoction,and western medicine groups(P＞0.05).After 6 weeks of intervention,both the Jianpi Zhidong Decoction and western medicine groups had lower body weight than the model group(P＜0.05).Behavioral scores showed no significant differences between the model,Jianpi Zhidong Decoction,and western medicine groups(P＞0.05).After 6 weeks of intervention,the Jianpi Zhidong Decoction and western medicine groups had lower scores than the model group(P＜0.01).Compared with the blank group,the model group showed lower serum HA levels(P＜0.05).However,serum HA levels were higher in the Jianpi Zhidong Decoction group than in the model group(P＜0.05),while the western medicine group showed a non-significant increase(P＞0.05).Striatal HA levels were lower in the model group than in the blank group(P＜0.05),but higher in the Jianpi Zhidong Decoction and western medicine groups than in the model group(P＜0.01).Striatal HDC mRNA ex-pression was lower in the model group than in the blank group but the difference was not significant(P＞0.05).Compared with the model group,the Jianpi Zhidong Decoction and western medicine groups showed the higher striatal HDC mRNA expression,but the difference was not significant(P＞0.05).Western blot results indicated that striatal HDC protein expression was lower in the model group than in the blank group(P＜0.05),while it was higher in the Jianpi Zhidong Decoction group than in the model group(P＜0.05)and the western medicine group(P＞0.05).Immunohistochemistry showed that the IOD value of HDC in the striatum was low-er in the model group than in the blank group(P＜0.05).Compared with the model group,the IOD value of HDC in the striatum was higher in the Jianpi Zhidong Decoction group(P＜0.05)and the western medicine group(P＞0.05).Conclusion:Jianpi Zhidong Decoction can increase serum and striatal HA levels and striatal HDC expression in TS rats.
Jianpi Zhidong DecoctionWood-inhibiting and earth-reinforcingRat,Tourette syndromeSerumStriatumHista-mine decarboxylaseHistamine
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