首页|土大黄提取物对银屑病小鼠P物质及其神经肽受体-1表达的影响

土大黄提取物对银屑病小鼠P物质及其神经肽受体-1表达的影响

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  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
目的:经土大黄干预,探讨P物质(SP)及其神经激肽-1受体(NK-1R)在银屑病皮肤组织中的表达及意义.方法:收集对照组,银屑病模型组,甲氨蝶呤片组(1.3 mg/kg),阿瑞匹坦胶囊组(5 mg/kg),土大黄组(1、2、4 g/kg)小鼠皮肤、血液标本,经苏木精-伊红(HE)染色,观察银屑病小鼠皮肤组织病理学变化;采用实时荧光定量聚合酶链反应(RT-qPCR)技术,检测银屑病小鼠皮肤SP、NK-1R mRNA表达;采用酶联免疫吸附测定、蛋白免疫印迹技术,检测银屑病小鼠血液SP、皮肤、NK-1R蛋白表达;随后分析SP、NK-1R表达水平与银屑病的关系.结果:与银屑病模型组比较,所有药物干预组均能改善银屑病小鼠皮肤组织病理学变化;同时,小鼠皮肤SP、NK-1RmRNA表达,血清SP、皮肤NK-1R蛋白表达均降低(P＜0.05,P＜0.01).与阳性对照药甲氨蝶呤片组、阿瑞匹坦胶囊组比较,1 g/kg大黄组小鼠皮肤组织NK-1R蛋白表达升高(P＜0.05).与土大黄组(1 g/kg)比较,土大黄组(2、4 g/kg)小鼠皮肤组织NK-1R蛋白表达均降低,其中土大黄组(2 g/kg)(P＞0.05)、土大黄组(4 g/kg)(P＜0.05).结论:土大黄可能通过降低焦虑相关神经递质SP及其NK-1R受体基因、蛋白表达,从而改善小鼠银屑病样皮损,其作用不亚于阳性对照药甲氨蝶呤片、阿瑞匹坦胶囊.
Effect of Rumex obtusifolius L.Extract on Substance P and Neurokinin-1 Receptor Expression in Psoriasis Mice
Objective:To investigate the expression and significance of substance P(SP)and its neurokinin-1 receptor(NK-1R)in psoriatic skin tissue after intervention with Rumex obtusifolius L.Methods:Skin and blood samples were collected from control mice,psoriasis model mice,and mice treated with methotrexate tablets(1.3 mg/kg),aprepitant capsules(5 mg/kg),and R.obtusi-folius at doses of 1,2,and 4 g/kg.Hematoxylin and eosin(HE)staining was used to observe the pathological changes in psoriatic skin tissues.Real-time fluorescence quantitative polymerase chain reaction(RT-qPCR)was employed to detect the expression of SP and NK-1R mRNA in psoriatic skin.Enzyme-linked immunosorbent assay(ELISA)and Western blot were used to measure the ex-pression of SP in serum and NK-1 R protein in the skin.Subsequently,the relationship between the expression levels of SP,NK-1 R,and psoriasis was analyzed.Results:Compared to the psoriasis model group,all drug intervention groups showed improvements in the pathological changes of psoriatic skin tissue and reduced expression of SP and NK-1 R mRNA in the skin,and SP in serum,as well as NK-1 R protein in the skin(P＜0.05,P＜0.01).Compared to the positive control drugs methotrexate and aprepitant,the R.obtusifolius group at 1 g/kg showed increased expression of NK-1 R protein in the skin tissue(P＜0.05).Compared to the results in the R.obtusifolius group at 1 g/kg,the expression of NK-1R protein in the skin tissue was decreased in the R.obtusifolius groups at 2 g/kg and 4 g/kg,with the 2 g/kg group showing a non-significant decrease(P＞0.05)and the 4 g/kg group showing a signifi-cant decrease(P＜0.05).Conclusion:R.obtusifolius may improve psoriasis-like skin lesions in mice by reducing the expression of the anxiety-related neurotransmitter SP and its receptor NK-1R at both the gene and protein levels.Its effectiveness is comparable to the positive control drugs methotrexate and aprepitant.

Rumex obtusifolius LPsoriasisMiceHE stainingRT-qPCRElisaWestern blotSubstance PNeurokinin-1 re-ceptor

热比姑丽·伊斯拉木、优力都孜·买买提、玉素甫江·艾力、开丽比努尔·阿布来提、巴合沙拉·马乃甫

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新疆维吾尔自治区维吾尔医药研究所,乌鲁木齐,830000

新疆维吾尔医方剂学实验室,乌鲁木齐,830000

新疆医科大学维吾尔医学院,乌鲁木齐市,830000

土大黄 银屑病 小鼠 苏木精-伊红染色 实时荧光定量聚合酶链反应 酶联免疫吸附测定 蛋白免疫印迹 P物质 神经肽-1受体

国家自然科学基金项目新疆维吾尔自治区自然科学基金面上项目新疆维吾尔自治区卫生健康委青年科技人才专项新疆维吾尔自治区公益性科研院所专项新疆维吾尔自治区公益性科研院所专项新疆维吾尔自治区重点实验室维吾尔医方剂学实验室开放课题

810603142021DO1A1582015Y40KY2015138KY2015139XJYS1104-2012-04

2024

10.3969/j.issn.1673-7202.2024.15.009

世界中医药
世界中医药学会联合会

世界中医药

CSTPCDCHSSCD北大核心
影响因子：1.266
ISSN：1673-7202
年,卷(期)：2024.19(15)