Regulatory Effect of Fanlian Huazhuo Formula on Cholesterol Metabolism Disorder in Acute Hyperlipidemia Mice
Objective:To investigate the regulatory effect of Fanlian Huazhuo Formula on cholesterol metabolism disorder in mice with acute hyperlipidemia.Methods:An acute hyperlipidemia model was established in C57BL/6 mice using Triton-WR1339.After 5 days of treatment,serum levels of total cholesterol(TC),triglycerides(TG),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),glutamic oxalacetic transaminase(GOT),and glutamic-pyruvic transaminase(GPT)were measured.Hematoxylin-eosin(HE)staining was used to observe pathological changes in the liver.Oil Red O staining was per-formed to detect lipid accumulation in the liver.Western blot was used to detect changes in the expression of asialoglycoprotein re-ceptor 1(ASGR1),liver X receptor α(LXRα),ATP-binding cassette transporter A1(ABCA1),ABCA5,and cytochrome P450 7A1(CYP7A1)in the liver.Results:Compared with the model group,Fanlian Huazhuo Formula significantly reduced serum levels of TC,TG,LDL-C,GOT,and GPT,increased HDL-C levels(all P＜0.05),significantly upregulated the protein expression levels of ABCA1,ABCG5,LXRα,and CYP7A1,significantly downregulated the protein expression level of ASGR1(all P＜0.05),signifi-cantly reduced lipid accumulation in hepatocytes,and improved liver pathology.Conclusion:Fanlian Huazhuo Formula may pro-mote cholesterol efflux and reduce blood lipids in mice with acute hyperlipidemia by regulating the ASGR1/LXRα/CYP7A1 signa-ling pathway.
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