Therapeutic Effect and Mechanism of Paeoniflorin on Scald Injuries in Rats Based on Network Pharmacology
Objective:To investigate the therapeutic effect of paeoniflorin on scald injuries in rats and explore the underlying mecha-nisms of paeoniflorin in burn treatment based on network pharmacology and molecular docking techniques.Methods:A deep second-degree scald model was established in rats.The effect of paeoniflorin on wound appearance and histopathological changes in wound tis-sues were observed,and wound healing rate was calculated.Relevant targets of paeoniflorin and burn injuries were identified using da-tabases such as PubChem,PharmMapper,and GeneCards.The STRING database and Cytoscape software were utilized to construct and visualize a multi-level molecular network of Chinese medicine(compound)-target-disease.Core genes were screened through MCODE analysis,followed by Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analyses.Finally,mo-lecular docking validation was performed using AutoDock Vina.Results:Paeoniflorin significantly promoted wound healing in scalded rats,enhanced angiogenesis and new epithelial formation,and reduced local inflammatory cell infiltration.A total of 337 potential chemical targets of paeoniflorin were identified,with 138 common targets related to burn injuries,including matrix metalloproteinase 1(MMP1)and tumor necrosis factor(TNF).Enrichment analysis indicated that the core targets of paeoniflorin in burn treatment were primarily involved in the PI3K/AKT signaling pathway,MAPK signaling pathway,etc.Molecular docking results showed that paeoni-florin exhibited good binding activity with key targets such as protein kinase B1(AKT1),albumin(ALB),interleukin-1 β(IL-1 β),TNF,and vascular endothelial growth factor A(VEGFA).Conclusion:Paeoniflorin exerts therapeutic effects on burns by inhibiting the inflammatory response,regulating early microcirculation,and promoting angiogenesis.This study confirms the therapeutic potential of paeoniflorin in burn treatment and elucidates the related molecular mechanisms.
PaeoniflorinBurnsNetwork pharmacologyMolecular dockingMechanism of actionAnimal experimentSignaling pathwayEnrichment analysis
万方数据
维普
