摘要
目的:研究通关藤水煎液中抗骨肉瘤细胞活性成分.方法:采用大孔树脂柱层析、硅胶柱层析、制备型高效液相色谱(HPLC)、结合磁共振成像波谱(NMR)、高分辨率质谱(HR-MS)等方法,对通关藤水煎液的化学成分进行系统分离与结构鉴定;采用细胞计数检测(CCK-8)方法进行抗骨肉瘤细胞活性评价.结果:分离鉴定了 33个化合物.其中,化合物1～8、化合物24～28均为首次从通关藤中分得;化合物6、化合物9、化合物21、化合物22、化合物25、化合物26、化合物29～31均能显著抑制骨肉瘤细胞MG63、143B的增殖.结论:通关藤水煎液中多个成分具有抑制骨肉瘤细胞活性.研究结果为通关藤的应用及进一步开发提供了科学依据.
Abstract
Objective:To investigate the active constituents against osteosarcoma cells in Marsdeniae Tenacissimae Caulis(MTC)decoction.Methods:The chemical constituents in MTC decoction were investigated by macroporous resin column chromatography(CC),silica gel CC,preparative high performance liquid chromatography(HPLC),nuclear magnetic resonance(NMR)spectrosco-py,and high-resolution mass spectroscopy(HR-MS).Their anti-osteosarcoma activities were evaluated by the CCK-8 method.Re-sults:Thirty-three compounds were isolated and identified.Among them,compounds 1 to 8 and 24 to 28 were isolated from Mars-deniae Tenacissimae Caulis for the first time.Compounds 6,9,21,22,25,26,and 29 to 31 could significantly inhibit the prolifera-tion of osteosarcoma cells MG63 and 143B.Conclusion:Multiple components of MTC decoction had the inhibitory activities against osteosarcoma cells.This study provides a scientific basis for the application and further exploitation of MTC.
基金项目
国家自然科学基金项目(82003987)
上海市浦东新区卫健委科技项目卫生行业专项(PW2021E-03)
上海市浦东新区科技发展基金民生科研专项(PKJ2023-Y51)
内蒙古自治区"科技兴蒙"计划项目(2022XYJG0001-02-01)
维普
万方数据