Quality Evaluation of Codonopsis Radix and Its Formula Granules Based on HPLC Fingerprint
Objective:To establish high performance liquid chromatography(HPLC)fingerprint of Codonopsis Radix and its formu-la granules and a simultaneous content determination method of their index components and conduct a comprehensive quality evalu-ation.Methods:HPLC was employed to develop a simultaneous content determination method for syringin and lobetyolin and estab-lish a fingerprint.The Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine(Version 2012)was used to evaluate the similarity and determine the common peaks.Results:The established fingerprint and multi-index-compo-nent content determination method meet the requirements of methodological investigation,and the content of the two components is different in different degrees.A total of 25 common peaks were found in the fingerprint,and 2 of them were identified.The similarity between the fingerprints of 10 batches of Codonopsis Radix formula granules from different manufacturers and the control fingerprint was＞0.900.Conclusion:The fingerprint has strong characteristics and is easy to operate,which can be used for the quality control of Codonopsis Radix formula granules.
万方数据
维普
