Ameliorative Effects of Marine Polysaccharides on DSS-induced Ulcerative Colitis in Mice
Objective:To investigate the effects of polysaccharides(sodium alginate and fucoidan)from marine Chinese medicines on dextran sulfate sodium(DSS)-induced ulcerative colitis(UC)in mice.Methods:RAW264.7 cells were treated with different concentrations of sodium alginate and fucoidan,and cell viability was measured by CCK-8 assay.RAW264.7 cells were activated by lipopolysaccharide(LPS).ELISA kits were used to determine the content of NO and interleukin-6(IL-6)in the cell supernatant.Twenty-five female mice were randomly divided into blank control group,model group,positive control group,sodium alginate group,and fucoidan group,with 5 mice in each group.Except the blank control group,each group was treated with DSS by gavage for 7 days to establish a US model,and then the positive control group,the sodium alginate group,and the fucoidan group were trea-ted with postbiotics,sodium alginate,and fucoidan by gavage,respectively.Blood samples were collected from eyeballs for the detec-tion of serum inflammatory mediators.At the same time,mice were sacrificed under anesthesia,and colon tissues were obtained for biochemical indexes determination and histopathological analysis.Results:Sodium alginate and fucoidan did not cause toxicity to RAW264.7 cells even at high concentrations.In the concentration range of 500 to 1 000 μg/mL,both sodium alginate and fucoidan inhibited the secretion of NO and IL-6 in RAW264.7 cells in a concentration-dependent manner,and the effect of fucoidan was sig-nificantly better than that of sodium alginate.For DSS-induced UC in mice,sodium alginate and fucoidan can improve the clinical symptoms of UC,restore the body weight of mice,and reduce the secretion levels of serum inflammatory mediators tumor necrosis factor-α(TNF-α)and IL-6.Conclusion:Fucoidan and sodium alginate can treat DSS-induced UC by inhibiting the release of in-flammatory mediators in serum,and the therapeutic effect of fucoidan is better than that of sodium alginate.
Polysaccharides from marine Chinese medicineSodium alginateFucoidanUlcerative colitisLipopolysaccharideInflammatory mediatorDextran sulfate sodiumRAW264.7 cells
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维普
