Integrated Pharmacokinetics of Flavonoids from Different Processed Products of Scutellariae Radix
Objective:To compare the content differences of eight flavonoid components in different processed products of Scutel-lariae Radix(SR)in rats and to explore the differences of pharmacokinetic behaviors of these products,providing scientific evidence for elucidating the processing principles.Methods:Twenty SD rats were randomly divided into control group,raw SR group,wine-processed SR group,and carbonized SR group,with five rats in each group.Drugs were administered by gavage.Using kaempferol as an internal standard,ultra-high-performance liquid chromatography-triple quadrupole mass spectrometry(UPLC-QQQ-MS/MS)was employed to measure the concentrations of baicalin,5,7,2',5'-tetrahydroxy-8,6'-dimethoxyflavone(HQ-6),wogonoside,baicalein,wogonin,chrysin,5,6-dihydroxy-7,8,2',6'-tetramethoxyflavone(HQ-2),and oroxylin A in rat serum at various time points.The ar-ea under the curve(AUC0→∞)percentage of each component was used as a custom weighting coefficient to calculate the overall ser-um concentration of the drugs in rats and establish an integrated pharmacokinetic model to compare the differences in pharmacoki-netic parameters of flavonoid components in processed products of SR.Results:The AUC0→∞ for single components varied signifi-cantly.The integrated pharmacokinetic results of multiple components showed that after wine processing of SR,the mean retention time(MRT0 →∞)of the eight flavonoid components was reduced from 17.22 h to 13.13 h.The time to reach peak concentration(Tmax)decreased from 0.50 h to 0.25 h,while the time to secondary peak increased from 4 h to 8 h.After carbonization of SR,the overall MRT for the carbonized product was 10.07 h,which was not significantly different from the wine-processed product(P＞0.05).The carbonized SR showed three peaks at 0.25,1,and 2 h.Conclusion:This method is specific and highly sensitive,suit-able for simultaneous detection and pharmacokinetic studies of eight flavonoid components in rat serum,further elucidating the sci-entific meaning of"enhanced efficacy by wine processing and stability by carbonization'for SR.
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