Traditional Chinese Medicine in Treatment of Acute Lymphocytic Leukemia Based on Network Pharmacology and Experimental Study
Objective:To explore the mechanisms of traditional Chinese medicine(TCM)in the treatment of acute lymphoblastic leukemia(ALL)and to validate these findings through experiments.Methods:A literature review was conducted to collect the me-dicinal compositions used in TCM for ALL treatment.The Traditional Chinese Medicine Systems Pharmacology Database and Analy-sis Platform(TCMSP)and the UniProt database were utilized to gather high-frequency TCM components and their targets.Disease-related targets were obtained from the Gene Expression Omnibus(GEO)database,and intersection targets were identified.Gene Ontology(GO)enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were performed using the Microarray Analysis Platform.A protein-protein interaction(PPI)network was constructed using Cytoscape 3.9.1.Based on network pharmacology predictions,molecular docking and experimental validation were carried out.Results:Ten high-frequency TCMs were identified,comprising 157 active components and 280 targets,with 2 428 disease-related targets for ALL.After filtering,37 intersection targets were obtained.KEGG enrichment analysis indicated that these targets were involved in multiple cancer,immune,and inflammatory response-related signaling pathways,including interleukin-17(IL-17),chemokine re-ceptor activation,and T-cell receptor pathways.The core active component identified was quercetin,with the key target being protein kinase B(AKT1).Molecular docking results showed a good interaction between quercetin and AKT1.Cell experiments demonstra-ted that quercetin treatment significantly reduced the viability of ALL cells and increased apoptosis rates(P＜0.05),with a de-crease in the expression of phosphoinositide 3-kinase(PI3K)and phosphorylated protein kinase B(p-AKT).Conclusion:Prelimi-nary screening and predictions from network pharmacology indicate that TCM can treat ALL through multiple components,targets,and pathways.The core component quercetin may inhibit ALL cell proliferation and induce apoptosis by regulating the PI3K/AKT signaling pathway,providing a reference for the development and application of effective components in TCM for ALL treatment.
Acute lymphocytic leukemiaTraditional Chinese medicineActive ingredientsNetwork pharmacologyCell experi-mentsWeighted gene co expression network analysisMolecular dockingMechanism of action
万方数据
维普
