Mechanism of Asperuloside on Antioxidative Stress and Anti-apoptosis in Rats with Oligoasthenospermia
Objective:To investigate the mechanism of asperuloside(ASP)in combating oxidative stress and apoptosis in a rat model of oligoasthenospermia.Methods:A rat model of oligoasthenospermia was established by oral administration of ornidazole.SD rats were randomly divided into a control group,a model group,an ASP group(60 mg/kg),and pathway inhibitor group(intraperito-neal injection at 2 mg/kg in the 3rd week)+ASP group according to a random number table,with 10 rats in each group.Sperm pa-rameters were assessed.Serum levels of malondialdehyde(MDA),glutathione(GSH),superoxide dismutase(SOD),and glutathione peroxidase(GSH-Px)were measured by enzyme-linked immunosorbent assay(ELISA).Real-time quantitative polymerase chain re-action(qPCR)was used to detect gene expression levels of B-cell lymphoma 2(Bcl-2),Bcl-2-associated X protein(Bax),and nu-clear factor erythroid 2-related factor 2(Nrf2)in testicular tissue.Western blot was used to measure levels of Kelch-like ECH-asso-ciated protein 1(Keap1),Nrf2,and heme oxygenase-1(HO-1)in testicular tissue.Results:Compared with the control group,the model group showed decreased sperm motility,sperm concentration,GSH-Px,GSH,SOD,Bcl-2,Keap1,Nrf2,and HO-1 levels(P＜0.05),and increased MDA and Bax levels(P＜0.05).Compared with the model group,the ASP group demonstrated increased sperm motility,sperm concentration,GSH-Px,GSH,SOD,Bcl-2,Keap1,Nrf2,and HO-1 levels(P＜0.05),and decreased MDA and Bax levels(P＜0.05).Conclusion:ASP can effectively improve sperm motility and concentration in oligoasthenospermia rats,reduce apoptosis in testicular cells,and mitigate oxidative stress damage.Activation of the Keap1/Nrf2 signaling pathway may be the underlying mechanism of action.
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维普
