Mechanism of Qinjiao Biejia Powder in Improving Psoriasis Skin Lesions in Mice by Regulating the Toll-like Receptor 4 Signaling Pathway
Objective:To investigate the molecular mechanisms of Qinjiao Biejia Powder in improving skin lesions and inflammato-ry response in mice with psoriasis.Methods:Sixty mice were randomly divided into a control group,a model group,a methotrexate group(1 mg/kg),and low-dose,medium-dose,and high-dose Qinjiao Biejia Powder groups(0.98,1.95,and 3.90 g/kg,respective-ly)follow the random number table method.The control group only received hair removal,and the psoriasis mouse model was estab-lished using imiquimod in other groups.Hematoxylin-eosin(HE)staining was used to observe histopathological changes in the skin lesions.The psoriasis area and severity index(PASI)was used to assess the degree of skin lesions.Enzyme-linked immunosorbent assay(ELISA)measured serum levels of inflammatory mediators.Quantitative real-time PCR(qRT-PCR)detected mRNA expres-sion levels of interleukin-17(IL-17),interleukin-23(IL-23),interleukin-22(IL-22),interleukin-6(IL-6),and Toll-like receptor 4(TLR4).Immunohistochemistry assessed the expression of NOD-like receptor protein 3(NLRP3),and Western blot analyzed the expression levels of NLRP3 and proteins related to the TLR4/NF-κB pathway.Results:Compared with the model group,the metho-trexate group and the low-dose,medium-dose,and high-dose Qinjiao Biejia Powder groups showed significant improvement in skin lesions,notably reduced PASI scores,and significantly decreased serum levels of inflammatory mediators,as well as the expression of NLRP3 and TLR4/NF-κB pathway-related proteins(all P＜0.05).Conclusion:Qinjiao Biejia Powder can ameliorate skin le-sions and inflammatory responses in mice with psoriasis,likely through the inhibition of the TLR4/NF-κB pathway and downregula-tion of NLRP3 expression.
PsoriasisQinjiao Biejia PowderToll-like receptors 4Nuclear factor-κBNOD-like receptor protein 3Skin lesionInflammatory responsePsoriasis area and severity index scoring
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