Objective:To explore the mechanism of Shanjia Baihua Decoction combined with gemcitabine in combating drug resist-ance in pancreatic cancer.Methods:Thirty mice were randomly divided into a model group,a gemcitabine group,and a combination group.A subcutaneous tumor transplantation model was established in mice.Mice were treated with Shanjia Baihua Decoction,gem-citabine,or Shanjia Baihua Decoction and gemcitabine in combination.Changes in tumor volume and weight were observed,along with the steroid receptor coactivator(Src)/inhibitor of differentiation(Id1)signaling pathway proteins.The human pancreatic canc-er cell line PANC-1 was divided into control,gemcitabine treatment,combination treatment,combination+miR-124-3p inhibitor,and combination+overexpressed SDF2L1 groups.Cell proliferation and migration abilities,as well as levels of miR-124-3p,SDF2L1,and mRNA were compared.Results:Compared to the gemcitabine group,the combination group exhibited reduced tumor volume and weight,and decreased levels of Id1 and p-Src/Src,and SDF2L1.Compared to the control group,the gemcitabine treat-ment group showed significantly reduced cell proliferation and migration rates,increased miR-124-3p levels,and decreased SDF2L1 levels.Compared to the gemcitabine treatment group,the combination group further reduced cell proliferation and migration rates,elevated miR-124-3p,and decreased SDF2L1 levels.Compared to the combination group,the combination+miR-124-3p inhibitor group and the combination+overexpressed SDF2L1 group showed significantly increased cell proliferation and migration rates,and elevated SDF2L1 levels.Conclusion:Shanjia Baihua Decoction combined with gemcitabine exerts its anti-drug resistance effect in pancreatic cancer by upregulating miR-124-3p to inhibit SDF2L1.
维普
万方数据