Objective:To investigate the antitumor effect and mechanisms of tubeimoside Ⅰ(TBMS1)on A549 lung cancer cells.Methods:The study evaluated the effects of TBMS1 on the viability and proliferation of A549 lung cancer cells using a cell count-ing kit(CCK)assay and colony formation assay.The influence of TBMS1 on autophagic flux in A549 cells was analyzed through Western blot(WB),with immunofluorescence used for validation.Additionally,WB was employed to detect the mammalian target of rapamycin(mTOR)signaling pathway,a core autophagy pathway,to preliminarily explore the underlying mechanisms.Results:TBMS1 significantly inhibited the viability of A549 cells in a concentration-dependent manner.The colony formation assay demon-strated that TBMS1 reduced the proliferation of A549 cells and suppressed colony formation.WB results showed that TBMS1 in-creased the formation of microtubule-associated protein light chain 3(LC3)and induced autophagy in A549 cells,while promoting the degradation of sequestosome 1(SQSTM1).These findings suggest that TBMS1 may promote autophagy via the mTOR signaling pathway.Conclusion:TBMS 1 inhibits the proliferation of A549 lung cancer cells by inducing autophagy and promoting the degrada-tion of SQSTM1.Its mechanism likely involves the inhibition of mTOR activity,leading to impaired autophagic flux.
关键词
土贝母皂苷甲/肺癌/肺癌细胞A549/自噬/哺乳动物雷帕霉素信号通路/自噬流/螯合体1/增殖
Key words
Tubeimoside Ⅰ/Lung cancer/A549 lung cancer cells/Autophagy/Mammalian target of rapamycin signaling path-way/Autophagic flow/SQSTM1/Proliferation
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