首页|土贝母皂苷甲对肺癌细胞A549的抗肿瘤作用及机制研究

土贝母皂苷甲对肺癌细胞A549的抗肿瘤作用及机制研究

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目的:探究土贝母皂苷甲(TBMS1)对肺癌细胞A549的抗肿瘤作用及机制.方法:本研究通过细胞增计数剂盒和细胞集落形成试验检测了 TBMS1对肺癌细胞A549细胞株细胞活性和增殖能力的影响;通过蛋白质免疫印迹法(WB)实验检测了 TBMS1对A549细胞株自噬流的影响,并采用免疫荧光对结果进行了验证;WB实验对自噬核心通路哺乳动物雷帕霉素(mTOR)信号通路进行检测,对其机制进行初步分析.结果:TBMS1能够显著抑制肺癌细胞A549的细胞活力,并且呈现出浓度依赖性的抑制作用;集落形成试验结果显示,TBMS1可以抑制肺癌细胞A549的增殖,减少集落的形成;WB检测结果显示TBMS1增加了微管相关蛋白轻链3(LC3)的形成,诱导了肺癌细胞A549的自噬,并促进了螯合体1(SQSTM1)的降解;TBMS1可能是通过mTOR信号通路促进了细胞自噬.结论:TBMS1通过诱导肺癌细胞A549自噬,促进SQSTM1的降解,抑制了细胞增殖,其机制可能是抑制了 mTOR的活性,导致细胞的自噬流受阻.
Antitumor Effect and Mechanisms of Tubeimoside Ⅰ on A549 Lung Cancer Cells
Objective:To investigate the antitumor effect and mechanisms of tubeimoside Ⅰ(TBMS1)on A549 lung cancer cells.Methods:The study evaluated the effects of TBMS1 on the viability and proliferation of A549 lung cancer cells using a cell count-ing kit(CCK)assay and colony formation assay.The influence of TBMS1 on autophagic flux in A549 cells was analyzed through Western blot(WB),with immunofluorescence used for validation.Additionally,WB was employed to detect the mammalian target of rapamycin(mTOR)signaling pathway,a core autophagy pathway,to preliminarily explore the underlying mechanisms.Results:TBMS1 significantly inhibited the viability of A549 cells in a concentration-dependent manner.The colony formation assay demon-strated that TBMS1 reduced the proliferation of A549 cells and suppressed colony formation.WB results showed that TBMS1 in-creased the formation of microtubule-associated protein light chain 3(LC3)and induced autophagy in A549 cells,while promoting the degradation of sequestosome 1(SQSTM1).These findings suggest that TBMS1 may promote autophagy via the mTOR signaling pathway.Conclusion:TBMS 1 inhibits the proliferation of A549 lung cancer cells by inducing autophagy and promoting the degrada-tion of SQSTM1.Its mechanism likely involves the inhibition of mTOR activity,leading to impaired autophagic flux.

Tubeimoside ⅠLung cancerA549 lung cancer cellsAutophagyMammalian target of rapamycin signaling path-wayAutophagic flowSQSTM1Proliferation

王茜、赵凡尘、吕祥、吴建春、张博、王媛媛、方志红、李雁

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上海中医药大学附属市中医医院肿瘤科,上海,200071

河南中医药大学中医药科学院,郑州,450046

土贝母皂苷甲 肺癌 肺癌细胞A549 自噬 哺乳动物雷帕霉素信号通路 自噬流 螯合体1 增殖

2024

世界中医药
世界中医药学会联合会

世界中医药

CSTPCDCHSSCD北大核心
影响因子:1.266
ISSN:1673-7202
年,卷(期):2024.19(19)