Effect of Modified Qihuang Decoction on Apoptosis of Renal Tissue in Rats with Membranous Nephropathy
Objective:To explore the effect of modified Qihuang Decoction on renal function and renal tissue cell apoptosis in rats with membranous nephropathy(MN).Methods:The MN rat model was established by an improved Border method,and rats were randomly divided into five groups and treated for 4 consecutive weeks.The blank group(20 rats)and the model group(15 rats)were administered 5 mL of physiological saline by gavage.The Benazepril group(18 rats)was administered benazepril hydrochlo-ride at 10 mg/kg by gavage.The low-dose and high-dose modified Qihuang Decoction groups(18 rats each)were administered modified Qihuang Decoction at 4 mg/kg and 8 mg/kg by gavage,respectively.Urine protein levels were measured at the end of weeks 2 to 6.At the end of week 6,renal pathological changes were observed by hematoxylin-eosin(HE)staining.The expression levels of Bcl-2-associated X protein(Bax),B-cell lymphoma 2(Bcl-2),and Wilms'tumor suppressor gene 1(WT-1)in renal tissue were detected by immunohistochemistry.Blood samples were collected from the abdominal aorta to measure blood urea nitrogen(BUN),serum creatinine(Scr),human serum albumin(Alb),triglycerides(TG),and total cholesterol(TC).Results:In the model group,24-hour urine protein,BUN,Scr,TG,TC,and Bax protein expression levels were significantly higher,while Bcl-2,WT-1,and Alb expression levels were significantly lower compared with the blank group(P＜0.01).In each treatment group,24-hour urine protein,BUN,Scr,TG,TC,and Bax protein expression levels were significantly lower,while Bcl-2,WT-1,and Alb expression levels were significantly higher compared with the model group(P＜0.05,P＜0.01).Furthermore,the high-dose modified Qihuang De-coction group showed more significant changes than the low-dose group.Conclusion:Modified Qihuang Decoction may protect podocytes in MN rats by reducing Bax protein expression,increasing Bcl-2 and WT-1 protein expression,and influencing cell apop-tosis,thereby delaying renal function impairment in MN.
万方数据
维普
