Effect of Amygdalin on Pulmonary Histopathology and Th17/Treg Immune Balance in Asthmatic Mice
Objective:To investigate the mechanism of action of amygdalin in modulating airway inflammation in an asthma model by observing its effect on pulmonary histopathology and the Helper T helper 17 cell(Th17)/regulatory T cell(Treg)immune bal-ance in asthmatic mice.Methods:Fifty SPF-grade BALB/c mice were randomly divided into five groups;blank control group,model group,dexamethasone group,low-dose amygdalin group,and high-dose amygdalin group.A mouse model of bronchial asthma was established by sensitizing BALB/c mice via intraperitoneal injection of ovalbumin(OVA)and aluminum hydroxide,followed by aerosolized inhalation.After the final challenge,treatment was administered for 7 days before tissue collection.Lung tissue pathology was observed using hematoxylin-eosin(HE)staining.Levels of inflammatory mediators,interleukin-10(IL-10)and IL-17,in the left lung bronchoalveolar lavage fluid(BALF)were measured by enzyme-linked immunosorbent assay(ELISA).Results;Pathological examination of the lung tissue showed that the blank control group had normal bronchiolar walls with no significant inflammatory cell infiltration around the bronchioles.In the model group,bronchiolar walls were significantly thickened with marked infiltration of inflammatory cells in the lumen and surrounding tissues.The dexamethasone,high-dose,and low-dose amygdalin groups showed var-ying degrees of inflammatory cell infiltration around the bronchioles,with less noticeable thickening of the bronchial walls.ELISA results showed that the IL-10 level in the model group was significantly lower than in the blank control group,and the IL-17 level was significantly higher(P＜0.01).Compared to the model group,all treatment groups showed an increase in IL-10 and a decrease in IL-17 levels(P＜0.01).Both the low-dose and high-dose amygdalin groups exhibited higher IL-10 and IL-17 levels compared to the dexamethasone group(P＜0.01).Conclusion;Both low and high doses of amygdalin can reduce lung tissue inflammation in the bronchial asthma model,decrease IL-17 secretion,and increase IL-10 levels,with effects superior to those of dexamethasone.
万方数据
维普
