Non-spherical colloidal particles had important research value in fields such as condensed matter physics and the development of new intelligent materials.Among them,square thin colloidal particles,which could be periodically and densely packed,had attracted widespread attention due to their simple shape,and diverse self-assembled structures.The material of the square thin colloidal particles prepared by photolithography was SU-8 epoxy resin.The side length of the particles was 2.3 μm and the thickness of the particles was 1.2 µm.Vertices were slightly rounded due to the influence of lithography accuracy.By analyzing the infrared absorption spectra of particles before and after photolithography,it was found that,after photolithography,the cross-linking degree of SU-8 epoxy resin inside the particles was relatively high.By calculating the relationship between the interaction potential of particles and the distance between particles,it was proven that the interaction between particles was a near hard-sphere interaction.Furthermore,a new particle vertex recognition algorithm was proposed based on the single-particle motion images of the square thin colloidal particles on both planar and spherical surfaces,which could avoid particle misrecognition problems caused by unclear images.