Comparison of Rhizosphere Microbial Characteristics of Five Different Bonsai Plants
The polymerase chain reaction-denaturing gradient gel electrophoresis(PCR-DGGE)method was used to determine the rhizosphere microorganism diversity of five bonsai species including Syzygium grijsii,Pyracantha fortuneana,Acer buergerianum,Acer palmatum,and Daphne odora.The results showed that the rhizosphere microorganism had significant differences among the five bonsai species.Electrophoresis lanes obtained by PCR-DGGE were able to reflect the difference.Soil microorganism diversity were higher in Acer palmatum and Acer pal matum,with peak diversity index of 3.121 and 2.876,they also had a higher similarity of rhizosphere microbial community.The lowest diversity index was observed in Pyracantha fortuneana,and the value was 1.038.These differences in rhizosphere microbial communities may be caused by root exudate secreted from different bonsai plants.Higher similarity of rhizosphere microbial community from Acer palmatum and Acer palmatum may be due to their close phylogenetic position(Aceraceae,Acer).A lower rhizosphere microbial diversity in Pyracanthafortuneana may be due to the root exudate secreted by Rosaceae plant,which contained substances triterpene acids and had inhibitory effect on microorganism.