Polyvinyl alcohol(PVA)/gelatin based materials had good film forming properties and biocompatibility,and had widely practical application value,which was an important research target.In this study,microcrystalline cellulose(MCC)and PVA were added to gelatin(GL)substrate as reinforcing materials and blending materials,respectively,to improve the mechanical properties and barrier properties of gelatin matrix composite films.The addition of microcrystalline cellulose improved the physicochemical properties and application properties of MCC/PVA/GL composite films.When 5%MCC is added to the substrate,the tensile strength of the composite film increases from 14.91 MPa to 48.02 MPa,the thermal degradation temperature increases by 11.4 ℃,and the water content decrease by 9.12%.At the same time,the path of water vapor through the PVA/GL film is prolonged,and the water vapor transmission rate of the MCC/PVA/GL composite film is reduced.Soil embedding experiments show that the addition of MCC accelerates the biodegradability of the composite films.Therefore,MCC/PVA/GL composite film can be applied to the field of green packaging,such as,food packaging,pharmaceutical packaging,cosmetic packaging and so on.