Effects of artificial feeding on the composition of gut microbiome of long-tailed hamster
The alteration in diet is a pivotal factor contributing to modifications in the gut microbiota of small mammals.When transitioning from their natural habitat to captivity,wild animals undergo drastic changes in their lifestyle,howev-er,the succession processes underlying the establishment of intestinal microbial community structure from artificial feed-ing remain incompletely elucidated.To clarify the effects of artificial feeding on the composition of gut microbiome of small mammals,natural populations of long-tailed hamster(Cricetulus longicaudatus)were captured using cage trapping from Lishi District(LS),Xi County(XX)and Zuoquan County(ZQ)in Shanxi Province in summer(from July to Sep-tember)of 2022.Based on 16S rDNA high-throughput sequencing,we analyzed the differences of intestinal flora in dif-ferent ecological environments,and explored the succession process of artificial feeding on the gut microbial composi-tion.The results showed that Firmicutes were significantly enriched in XX and ZQ populations while bacteroides were significantly enriched in LS population.Intestinimonas is a common dominant genus in the three populations.After 35 days of artificial feeding,Bacteroides were significantly enriched in the three populations and their relative abundance tended to be consistent.The relative abundance of Muribaculacea in ZQ population was 48.85％,which was significant-ly higher than that in the wild environment.Our study shows that the diversity of the gut microbiota of C.longicaudatus is different in distinct ecological environments.After long-term artificial feeding,the similarity of gut microbiome of dif-ferent C.longicaudatus populations increased significantly,indicating that diet change has a great impact on the diversi-ty and community composition of gut microbiome,and the composition of gut microbiome of C.longicaudatus may play an important role in nutrition metabolism.The results provided a scientific basis for a better understanding of the ecological adaptability of the intestinal microbiome of C.longicaudatus,and the utilization of food as a means to regu-late the population of detrimental.
国家科技期刊平台
NSTL
万方数据
