Effect of soybean isoflavones on growth performance,immune function,testicular development,and plasma reproductive hormone of Bashang long-tail chickens
The experiment was conducted to investigate the effect of soybean isoflavones on the growth performance,immune function,testicular development,and reproductive hormone of Bashang long-tail chickens.A total of 750 Bashang long-tail chickens aged 40 weeks were randomly divided into five groups with three replicates in each group and 50 chickens in each replicate.The diets of the group Ⅰ,group Ⅱ,group Ⅲ,group Ⅳ,and group Ⅴ were supplemented with 5,10,15,20,and 25 mg/kg soybean isoflavone,respectively.The pre-feeding period lasted for seven days,and the formal experimental period lasted for 63 days.The results showed that the weight gain of Bashang long-tail chickens in the group Ⅱ,group Ⅲ,group Ⅳ,and group Ⅴ was significantly higher than that in the group Ⅰ(P<0.05).The thymus index of the Bashang long-tail chickens in the group Ⅲ was significantly higher than that of other groups(P<0.05).The burse index of the Bashang long-tail chickens in the group Ⅲ was significantly increased by 16.09%,14.70%,and 22.67%compared to the group Ⅰ,group Ⅱ,and group Ⅴ,respectively(P<0.05).The spleen index of the Bashang long-tail chickens in the group Ⅲ was significantly increased by 17.98%compared to the group Ⅰ(P<0.05).The testicular index of the Bashang long-tail chickens in the group Ⅲ was the highest,which is significantly higher than the group Ⅰ(P<0.05),with an increase of 11.74%.The sperm collection volume,sperm density,and effective sperm count of the Bashang long-tail chickens in the group Ⅲ were significantly higher than those in the group Ⅰ(P<0.05),with the increase of 9.52%,4.79%,and 8.97%,respectively.The TESTO content of the Bashang long-tail chickens in the group Ⅲ was significantly increased by 10.42%,4.72%,and 6.42%compared to the group Ⅰ,group Ⅱ,and group Ⅴ,respectively(P<0.05).The LH levels of the Bashang long-tail chickens in the group Ⅲ significantly increased by 7.63%,5.83%,and 10.12%compared to the group Ⅰ,group Ⅱ,and group Ⅴ,respectively(P<0.05).The study indicates that soybean isoflavone can obviously improve the growth performance,immune function,testicular development,semen quality,and plasma reproductive hormone secretion level of Bashang long-tail chickens,and the optimal amount of additive is 15 mg/kg.