Effect of dietary replacing fish meal with compound protein source on growth performance,feed utilization,and immune performance of Litopenaeus vannamei
The study was to investigate the effect of replacing fish meal with a compound protein source(pig hemoglobin powder,corn protein powder,and enzymolysis defatted black soldier fly powder)in feed on the growth performance,feed utilization,immune performance,and ammonia resistance of Litopenaeus vannamei.A total of 1 440 healthy and active Litopenaeus vannamei with an initial weight of(1.01±0.01)g were randomly divided into six groups,with four replicates in each group,and 60 Litopenaeus vannamei in each replicate.0,20%,40%,60%,80%,and 100%of fish meal were replaced by compound protein sources with equal nitrogen and equal fat,respectively,named as the M1 group,M2 group,M3 group,M4 group,M5 group,and M6 group.The experiment lasted for 56 days.The results showed that the weight gain rate and specific growth rate of Litopenaeus vannamei in the M4 group were significantly higher than the M1 group(P<0.05).The feed efficiency of Litopenaeus vannamei in the M2 group,M3 group,and M4 group was significantly higher than the M1 group(P<0.05).The activity of aspartate aminotransferase(AST)and alanine aminotransferase(ALT),as well as the contentrations of total cholesterol(CHO),alanine aminotransferase(TG)in serum of Litopenaeus vannamei in the M6 group were significantly higher than the M1 group(P<0.05),and the activity of alkaline phosphatase(AKP)and total protein(TP)content in serum of Litopenaeus vannamei in the M6 group was significantly lower than the M1 group(P<0.05).The activity of glutathione peroxidase(GSH-Px),superoxide dismutase(SOD),and acid phosphatase(ACP)in the hepatopancreas of Litopenaeus vannamei in the M6 group were significantly decreased compared to other groups(P<0.05),and the content of MDA was significantly higher than that in the M1 group,M2 group and M3 group(P<0.05).After an ammonia challenge for 18 hours,the survival rate of Litopenaeus vannamei in the M6 group was significantly lower than that in other groups(P<0.05).The study indicates that the growth performance of Litopenaeus vannamei improved significantly when the fish meal is replaced by a compound protein source at a ratio of 60%,and that compound protein source to replace fish meal proportion is too high(over 80%)will reduce the feed utilization,immunity,and anti-stress ability of Litopenaeus vannamei.
Litopenaeus vannameienzymolysis defatted black soldier fly powdercorn protein powderpig hemoglobin powderfish mealgrowth performanceimmune performance
万方数据
维普
