首页|双水相协同超声法提取枇杷叶中绿原酸的工艺研究

双水相协同超声法提取枇杷叶中绿原酸的工艺研究

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试验采用响应面法优化双水相协同超声法提取枇杷叶中绿原酸的工艺,探讨提取液中乙醇体积分数、超声温度、超声时间、磷酸氢二钾用量和超声功率对绿原酸提取率的影响.结果显示,双水相协同超声法提取枇杷叶中绿原酸最佳提取工艺条件为乙醇体积分数61%、超声温度61℃、超声时间25 min、磷酸氢二钾用量4.6 g,此条件下绿原酸提取率1.53%,与预测值相接近.研究表明,双水相协同超声法提取枇杷叶中绿原酸的提取工艺稳定可行,可为枇杷叶的资源化开发与利用提供参考.
Study on extraction of chlorogenic acid from loquat leaves by biphasic synergistic ultrasound-assisted
The experiment employed response surface methodology to optimize the process of biphasic synergistic ultrasound-assisted extraction of chlorogenic acid from loquat leaves.The study investigated the effects of the ethanol volume fraction,ultrasound temperature,ultrasound time,potassium dihydrogen phosphate dosage,and ultrasound power on the extraction rate of chlorogenic acid.The results showed that the optimal extraction conditions for chlorogenic acid from loquat leaves by the biphasic synergistic ultrasound-assisted extraction were an ethanol volume fraction of 61%,ultrasound temperature of 61℃,ultrasound time of 25 minutes,and potassium dihydrogen phosphate dosage of 4.6 g.Under these conditions,the extraction rate of chlorogenic acid was 1.53%,which closely matched the predicted value.The study indicates that the biphasic synergistic ultrasound-assisted extraction is an effective and stable method for extracting chlorogenic acid from loquat leaves,providing valuable insights for the resource development of loquat leaves.

loquat leaveschlorogenic acidbiphasic synergistic ultrasound-assistedresponse surface methodology

韦正、黄秀香、叶红霞、邵柳红、赖红芳

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河池学院,广西 河池 546300

广西蚕桑生态学与智能化技术应用重点实验室,广西 河池 546300

微生物及植物资源开发利用广西高校重点实验室,广西 河池 546300

枇杷叶 绿原酸 双水相协同超声法 响应面法

广西自然科学基金项目国家自然科学基金项目河池学院校级重点科研课题2021年河池学院高层次人才科研启动项目桂西北生物多样性与药用资源开发重点实验室建设项目

2017GXNSFAA19811821563010XJ2017ZD052021GCC018

2024

10.13557/j.cnki.issn1002-2813.2024.01.013

饲料研究
北京市营养源研究所

饲料研究

CSTPCD北大核心
影响因子：0.391
ISSN：1002-2813
年,卷(期)：2024.47(1)
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