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竹燕窝提取物的抑菌活性研究

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试验以竹燕窝(海绵胶煤炱菌)为材料开发新型饲料添加剂.采用正交试验确定竹燕窝提取物的最佳提取工艺条件.采用牛津杯法探讨竹燕窝乙醇提取物对3种革兰氏阳性菌[粪肠球菌(Enterococcus faecalis)、金黄色葡萄球菌(Staphylococcus aureus)、表皮葡萄球菌(Staphylococcus epidermidis)]和3种革兰氏阴性菌[鼠伤寒沙门氏菌(Salmonella typhimurium)、鸡沙门氏菌(Salmonella pullorum)、大肠杆菌(Escherichia coli)]的抑菌效果.结果表明,竹燕窝抑菌提取物的最优提取工艺为乙醇浓度85%、液料比5 mL/g、提取时间180 min、提取温度50℃.竹燕窝乙醇提取物对革兰氏阳性菌的抑菌效果优于革兰氏阴性菌.研究表明,试验明确了竹燕窝在抑菌方面的潜在应用价值,可为抑菌产品的开发提供参考.
Study on antibacterial activity of Scorias spongiosa extract
The experiment used Scorias spongiosa as the material,to develop a new limiting resistance feed additive.An orthogonal test was used to determine the optimum extraction conditions of Scorias spongiosa extract.The Oxford cup method was employed to investigate the antibacterial effects of ethanol extracts from Scorias spongiosa on three Gram-positive bacteria(Enterococcus faecalis,Staphylococcus aureus,and Staphylococcus epidermidis)and three Gram-negative bacteria(Salmonella typhimurium,Salmonella pullorum,and Escherichia coli).The results indicated that the optimal extraction process for Scorias spongiosa inhibitory extracts was an ethanol concentration of 85%,a liquid-to-material ratio of 5 mL/g,an extraction time of 180 minutes,and an extraction temperature of 50℃.The inhibitory effect of Scorias spongiosa ethanol extracts on Gram-positive bacteria was superior to that on Gram-negative bacteria.The study clarifies the potential application value of Scorias spongiosa in terms of antibacterial properties,providing reference for the development of antibacterial products.

Scorias spongiosaGram-positive bacteriaGram-negative bacteriaantibacterial activity

冯鹏、俞骏晨、王易鑫、贾玮、苏洋、汪瀚宇、廖菲、农向

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乐山师范学院生命科学学院,四川 乐山 614000

竹类病虫防控与资源开发四川省重点实验室,四川 乐山 614000

东北农业大学动物科学技术学院,黑龙江 哈尔滨 150030

竹燕窝 革兰氏阳性菌 革兰氏阴性菌 抑菌活性

乐山师范学院校级高层次人才启动项目乐山师范学院校级科研培育项目重点项目乐山师范学院校级科研培育项目2022年乐山师范学院创新创业学院资助项目乐山市科技局重点项目2022年大学生创新创业训练计划项目

RC2021013KYPY2024-00052022SSDJS009SC-DGZZ20221122ZDYJ0090S202210649156

2024

10.13557/j.cnki.issn1002-2813.2024.01.015

饲料研究
北京市营养源研究所

饲料研究

CSTPCD北大核心
影响因子：0.391
ISSN：1002-2813
年,卷(期)：2024.47(1)
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