试验旨在研制具有高抗原活性的猪链球菌2型(Streptococcus suis type 2,SS2)疫苗培养基.研究以SS2 CVCC60615株为试验对象,通过单因素试验、Plackett-Burman试验、最陡爬坡试验和响应面分析得到SS2疫苗培养基.培养基发酵验证结果显示,SS2疫苗培养基菌液最大活菌数为1.09×109 CFU/mL,是TSB培养基最大活菌数的2.41倍.测定SS2不同时间点的抗原活性发现,在SS2发酵培养过程中当活菌数达到最大值后的1～3 h内细菌的抗原活性最高.小鼠感染试验表明,SS2抗原活性最高时制备的灭活疫苗与商品化灭活疫苗相比具有更高的免疫球蛋白G(IgG)抗体效价和疫苗保护率.研究表明,采用研制得到的高抗原活性SS2疫苗培养基制备的灭活疫苗具有IgG抗体效价高、免疫保护力强的优点.
Development and effect evaluation of Streptococcus suis type 2 vaccine medium with high antigenic activity based on response surface analysis
The study aimed to develop a Streptococcus suis type 2(SS2)vaccine medium with a high antigenic activity.SS2 CVCC60615 strain was used as the experimental object.The SS2 vaccine medium was developed by a single factor test,Plackett-Burman test,steepest ascent test and response surface analysis.The culture medium fermentation verification results showed that the maximum viable count in the culture medium of SS2 vaccine was 1.09×109 CFU/mL,which was 2.41 times higher than that obtained by using TSB medium.Through the determination of SS2 antigenic activity at different time points,it was found that the antigenic activity of bacteria was the highest within 1～3 h after the count of viable bacteria reached the maximum in the fermentation culture process of SS2.The mice infection test showed that the inactivated vaccine prepared when the SS2 antigen activity was the highest had a higher IgG antibody titer and a vaccine protection rate than the commercial inactivated vaccine.The study indicates that the inactivated vaccine prepared using the developed high antigen activity SS2 vaccine culture medium has the advantages of high IgG antibody titer and strong immune protection force.
Streptococcus suis type 2response surface analysishigh antigenic activityvaccine medium
万方数据
维普
