The study aimed to investigate the impact of astaxanthin on the hepatic health in juvenile Anguilla rostrata under histamine stress.Four hundred juvenile fish with an initial weight of(25.01±0.17)g were randomly divided into four groups(with four replicates per group and 25 fish per replicate):CK group,H group,H+AST4 group,and H+AST8 group.The juvenile fish were fed a basal feed,high histamine feed,and high histamine feed supplemented with 4 or 8 mg/kg of astaxanthin,respectively.The experiment lasted for 70 days.The results showed that compared to the CK group,the H group exhibited elevated serum AST and ALT activities(P<0.05).The hepatic SOD,CAT,GSH-Px activities,GSH concentration,and T-AOC decreased(P<0.05),while the MDA concentration increased in the H group(P<0.05).In the H+AST4 group and H+AST8 group,the serum AST and ALT activities were significantly lower than those in the H group(P<0.05),and the hepatic SOD,CAT,GSH-Px activities,GSH concentration,and T-AOC were higher than those in the H group(P<0.05),while the MDA concentration was lower than those in the H group(P<0.05).Compared to the CK group,the H group exhibited blurred cell boundaries,incomplete hepatic lobule structure,and numerous hepatocytes with nuclear displacement and vacuolar degeneration.The H+AST4 group and H+AST8 group showed clear cell boundaries,polygonal shapes,large and round nuclei centrally located,with no significant changes compared to the CK group.The study indicates that adding 4 mg/kg of astaxanthin to the feed under histamine stress can reduce serum aminotransferase activity and hepatic MDA concentrations,enhance hepatic antioxidant capacity,and improve hepatic structure,benefiting hepatic health.Adding 8 mg/kg of astaxanthin does not further improve the above indicators in juvenile Anguilla rostrata.
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