Effect of Bupleuri radix and its extract on growth,physiology and biochemistry,liver antioxidant,and antibacterial ability of juvenile koi carp
The experiment aimed to study the effect of Bupleuri radix and its extract on the growth,physiology and biochemistry,liver antioxidant,and antibacterial ability of juvenile koi carp.A total of 360 juvenile koi carp with an initial body weight of(21.0±0.5)g were randomly divided into three groups with three replicates in each group and 40 juvenile koi carp in each replicate.The juvenile koi carp in the group K,group C,and group T were fed a basal diet,a basal diet + 5‰ Bupleuri radix meal,a basal diet + 5‰ Bupleuri radix extract.The experiment lasted for 14 days.The results showed that in the serum of juvenile koi carp,the activity of lysozyme(LZM)in the group C and alkaline phosphatase(AKP)in the group C and group T at 14 days were significantly higher than those at 0 day,and significantly higher than those in the group K(P<0.05).In the liver of juvenile koi carp,the activity of LZM,acid phosphatase(ACP)in the group C and AKP in the group T at 14 days were significantly higher than those at 0 day(P<0.05).The activity of superoxide dismutase(SOD)and the total antioxidant capacity(T-AOC)in the group C at 14 days were significantly higher than those at 0 day(P<0.05),and the content of malondialdehyde(MDA)in the group C was lower than that at 0 day and 7 days.In the challenge test,the mortality rate of juvenile koi carp in the group K within 24 hours was 41.7%.The mortality rate in the group C was 18.3%and the protection rate was 56%.The mortality rate in the group T was 21.7%and the protection rate was 48%.In the serum,the interleukin-13(IL-13)content in the group C at 48 hours was significantly higher than that at 24 hours and 96 hours(P<0.05),and the immunoglobulin M(IgM)content in the group C and group T at 48 hours was significantly higher than that at 24 hours(P<0.05).In the liver,the content of interleukin-4(IL-4),IL-13,and IgM in the group C at 48 hours were significantly higher than those at 24 hours,and the content of IL-13 in the group C was significantly higher than that of the group K(P<0.05).The experiment indicates that adding Bupleuri radix and its extract to the diet for 14 days can effectively improve the physiological and biochemical indexes and anti-Aeromonas vickeri ability of juvenile koi carp,but the action time and effect of the two are different,and the effect of Bupleuri radix is more prominent.
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