Exploring mechanism of action of Qizhi oral liquid improving immune function of poultry based on network pharmacology and molecular docking
The aim of the experiment was to explore the mechanism of Qizhi oral liquid in enhancing the immune function of poultry.The active ingredients and their targets of Astragalus and Ganoderma lucidum in the compound were collected by the traditional Chinese medicine systematic pharmacology database and analysis platform(TCMSP),and the immune targets were collected by the OMIM,TTD and GeneCards databases.The potential target of Qizhi oral liquid for the poultry immunity was obtained.The Chinese medicine-component-target-pathway-disease network diagram and protein interaction(PPI)network were constructed by Cytoscape 3.9.1 software,and GO enrichment and KEGG pathway analysis were performed on the targets.The molecular docking of active ingredients and core targets was verified by molecular docking technology.The results showed that the 30 components of Qizhi oral liquid correspond to 66 targets,and 329 targets of poultry immunization,including 17 intersection targets.The core PPI targets included nuclear factor κB inhibitor α(NFKBIA),interleukin-6(IL-6)and interferon γ(IFNG).There were 38 results of GO functional enrichment,including 33 biological process items,four molecular function items and one cell component item.KEGG pathway analysis showed that the intersection genes were mainly concentrated in the C-type lectin receptor signaling pathway,the FoxO signaling pathway and the toll-like receptor signaling pathway.The results of molecular docking showed that the key components had good binding activity with the core protein target,which proved the reliability of the prediction results of network pharmacology.The study indicates that Qizhi oral liquid can enhance the immune function of poultry through a multi-component,multi-target and multi-pathway.
万方数据
维普
