首页|癸酸锌的合成及其对金黄色葡萄球菌和大肠杆菌的抑菌机制研究

癸酸锌的合成及其对金黄色葡萄球菌和大肠杆菌的抑菌机制研究

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研究旨在以癸酸与硫酸锌为原料高效制备癸酸锌并探讨其抑菌作用及机制.试验采用皂化复分解法将癸酸与硫酸锌螯合生成癸酸锌并进行结构表征;以金黄色葡萄球菌和大肠杆菌为模型,采用二倍稀释法测定其最低抑菌浓度(MIC)和最低杀菌浓度(MBC),研究癸酸锌的抑菌活性;检测癸酸锌对金黄色葡萄球菌和大肠杆菌的细胞膜通透性、生物膜形成、DNA与蛋白质合成的影响,阐释癸酸锌的抑菌机制.结果显示,癸酸锌晶型结构好,粒径均一,对金黄色葡萄球菌和大肠杆菌均具有较强的抑菌效果,MIC分别为250、350 mg/L,对金黄色葡萄球菌抑菌作用更强.癸酸锌可通过时间依赖方式破坏金黄色葡萄球菌和大肠杆菌细胞膜通透性,抑制菌体生物膜的形成,从而发挥抑菌作用,但对大肠杆菌和金黄葡萄球菌的DNA和蛋白质合成影响不显著.研究表明,癸酸锌可通过抑制生物膜形成、破坏细胞膜通透性等途径对革兰氏阳性菌和革兰氏阴性菌表现出较强的抑菌活性,具有作为抗生素替代品应用于畜禽生产的潜力.
Synthesis of zinc caprylate and its inhibitory mechanism against Staphylococcus aureus and Escherichia coli
The research aimed to efficiently prepare zinc caprylate using caprylic acid and zinc sulfate as raw materials and explore its antibacterial effects and mechanisms.The experiment employed a saponification and complex decomposition method to chelate caprylic acid with zinc sulfate to generate zinc caprylate,followed by structural characterization.Staphylococcus aureus and Escherichia coli were chosen as models,and the minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)were determined by a two-fold dilution method to investigate the antibacterial activity of zinc caprylate.The study also examined the impact of zinc caprylate on the cell membrane permeability,biofilm formation,DNA,and protein synthesis of Staphylococcus aureus and Escherichia coli to elucidate its antibacterial mechanism.The results showed that zinc caprylate had a well-defined crystal structure with uniform particle size.It exhibited strong antibacterial effects against both Staphylococcus aureus and Escherichia coli,with the MIC values of 250 and 350 mg/L,respectively,and a stronger inhibitory effect on Staphylococcus aureus.Zinc caprylate disrupted the cell membrane permeability of Staphylococcus aureus and Escherichia coli in a time-dependent manner,inhibited the formation of bacterial biofilms,thus exerting antibacterial effects.However,the impact on DNA and protein synthesis in both Escherichia coli and Staphylococcus aureus was not significant.The study suggests that zinc caprylate demonstrates strong antibacterial activity against both Gram-positive and Gram-negative bacteria by inhibiting biofilm formation and disrupting cell membrane permeability.It holds potential as a substitute for antibiotics in livestock and poultry production.

zinc caprylatemedium chain fatty acidsStaphylococcus aureusEscherichia coliantibacterial activitylivestock and poultry production

高蕊、李柯洁、周佳乐、祁丽雯、黄小露、高雨僮、乔汉桢、王金荣

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河南工业大学生物工程学院,河南 郑州 450001

癸酸锌 中链脂肪酸 金黄色葡萄球菌 大肠杆菌 抑菌活性 畜禽生产

"十四五"国家重点研发计划河南工业大学创新基金支持计划专项河南工业大学青年骨干教师培育计划项目

2021YFD13003002020ZKCJ2521421204

2024

10.13557/j.cnki.issn1002-2813.2024.02.016

饲料研究
北京市营养源研究所

饲料研究

CSTPCD北大核心
影响因子：0.391
ISSN：1002-2813
年,卷(期)：2024.47(2)
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