The experiment aimed to explore the effects of autumn supplementary feeding on the serum biochemical indexes of Oula sheep at different age stages.A total of 24 healthy and similar-conditioned Oula ewes at the ages of 6,12,and 36 months were selected for each age group.The Oula sheep at each age stage were randomly divided into a grazing group(group Ⅰ)and a grazing+supplementary feeding group(group Ⅱ),with three replicates in each group and four sheep in each replicate.The pre-feeding period was 10 days and the formal experimental period was 60 days.The results showed that the average daily weight gain of the group Ⅱ was higher than that of the group Ⅰfor 6-month-old and 12-month-old sheep(P＜0.05).In 6-month-old sheep of the group Ⅱ,the serum E2 and GH levels were higher than those in the group Ⅰ(P＜0.05).In 12-month-old and 36-month-old sheep of the group Ⅱ,the ALP activity was higher than that in the group Ⅰ(P＜0.05).In 6-month-old sheep of the group Ⅱ,the serum GLB level was higher than that in the group Ⅰ(P＜0.05).In 12-month-old sheep of the group Ⅱ,the IL-2 level was higher than that in the group Ⅰ(P＜0.05).In 36-month-old sheep of the group Ⅱ,the levels of GLB,IgM,IL-4,and IL-6 were higher than those in the group Ⅰ(P＜0.05).In 6-month-old sheep of the group Ⅱ,the serum GSH-Px activity was higher than that in the group Ⅰ(P＜0.05).In 12-month-old and 36-month-old sheep of the group Ⅱ,the SOD activity was higher than that in the group Ⅰ(P＜0.05).The study suggests that autumn supplementary feeding promotes the rapid maturation of 6-month-old ewes,enhances the growth,metabolic capacity,immune function,and antioxidant ability at different age in Oula sheep.
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